Study of the effect of the wound dressing Polyvinyl alcohol nanofiber containing Eucalyptus globules extract on surgical wounds infected by staphylococcus aureus in a rat model

Background and Aim : : Wound infection is one of the frequent complications in patients with a surgical operation. The use of nanotherapeutic agents engineered within the nanoscale is one of the most promising strategies for overcoming the wound infection. This study aimed to investigate the effect of the wound dressing Polyvinyl alcohol(PVA) nano fiber-containing Eucalyptus globules against Staphyloccocus. areous in surgical wounds using a rat model.Methods : ۶۰ male Wistar rats were randomly divided into four groups of treatment by extract+PVA, PVA, Nitroforazon, and Normal saline. A circular incision was made on the dorsal inter-scapular region of each rat. Then rats were inoculated with ۱۰^۶ CFU of S. areous at the site of skin wound. Treatments and normal saline ( control) were applied once every two days during the experiment. Tissue specimens were weighted and homogenized in broth on days ۷ and ۱۴. colony counts were conducted after serial dilution preparation.Results : a load of bacteria in untreated and treated by extract+PVA groups on day ۷ were ۸.۳*۱۰^۷ and ۴*۱۰^۶ and on day ۱۴ were ۵.۳۷*۱۰^۶ and ۶.۷۲*۱۰^۵ respectively while in Nitroforazone group was ۲.۸*۱۰^۶ and ۳.۸۵*۱۰^۵ CFU/ml.Conclusion : the results of this study showed the wound dressing Polyvinyl alcohol nanofiber containing Eucalyptus globules extract on surgical wounds infected by staphylococcus aureus has an antimicrobial effect similar to standard treatment with traditional antibiotics. Therefore nanotherapeutic agents consider as a potential alternative instead of antibiotics.

کلیدواژه ها:

wound infection ، Nanoextract ، Eucalyptus globules