Identification of Brucella melitensis biovar ۲ of bovine and human origin in Markazi province

Background and Aim : Brucellosis is one of the common diseases of man and animals are caused by bacteria of the genus Brucella. The objective of this study was to identify fifteen Brucella isolates of bovine and human origin, the species causing brucellosis, from Markazi provice based on proliferating IS۷۱۱ gene using PCR and sequencing.Methods : In this study during the spring and summer of ۲۰۱۸, a total of ۱۰۰ samples from suspected human and animals brucellosis. The identification was carried out through conventional methods such as macro and microscopic morphological descriptions, enzymatic activity, biochemical profile, substrate use and sensitivity to dyes. Complementary genotypic characterization was carried out using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis .Results : The Fifteen isolated DNA was extracted by Salting out method and amplified based on IS۷۱۱ fragment u.sing PCR, a band ۷۳۱bp on ۱% agarose gel, confirmed and identified all sampels are brucella melitensis. The results sequences and blasting with the data base sequences in gene bank shown high identity with Brucella melitensis biovare ۲ like strain M۵-۹۰ M۲۵, ATCC۲۳۴۵۷ with %۱۰۰ similarityConclusion : It was concluded that the phenotypic and molecular identification of fifteen isolates as B. melitensis biovar ۲ could be achieved using conventional and molecular techniques with enough resolution power. The identification of these isolates to the biovar level in epidemiological terms will allow the use of this genetic method as reference in future research. This finding constitutes the main out broke species might be brucella melitensis biovar ۲ in markazi province

کلیدواژه ها:

Brucellosis ، Identification ، zoonosis ، IS۷۱۱ ، Markazi province