T-cell and B-cell epitopes vaccine design on S-Ag of ۲۰۱۹ novel coronavirus (SARS-CoV-۲) using Immunoinformatics approach

Background and Aim : Coronaviruses are a large group of viruses that can infect animals and humans and has caused a large number of deaths with thousands of confirmed cases worldwide. These discomforts can be range from mild to severe. People of all ages can be infected by the new coronavirus (۲۰۱۹-nCoV). Older people, and people with pre-existing medical conditions (such as asthma, diabetes, heart disease) appear to be more vulnerable to becoming severely ill with the virus. As regards, there is no drug for treatment, it seems to be the most effective way to prevent and treat vaccinations. The aim of this study was T-cell and B-cell epitopes vaccine design against SARS-CoV-۲ in based on In silico analysis to determine the most conserved B and T-cell epitopes of S protein that could be able to stimulate a remarkable immune response.Methods : Sequence of S protein was collected from protein databases (NCBI & Uniprot) and analyzed by In silico tools (IEDB – ABCpred) for recognizing the most conserved immunogenic epitopes to trigger the T and B cell immune response. These suitable epitopes were evaluated in terms of toxicity and allergenicity and immunogenicity with used of Toxinpred and AllerTop and Vaxigen servers, respectively. Finally, the chimeric protein as a novel epitope-based vaccine was designed and assayed in terms of ۲D and ۳D structures by Prabi garland & Swiss-model servers, respectively.Results : T-cell and B-cell epitopes vaccine design study was predicted the most immunogenic epitopes (LPLVSSQCV) and (FLVLLPLVS) to stimulate TCD۸ and TCD۴ respectively, by IEDB server, also was predicted a suitable epitope (YGFQPTNGVGYQ) by ABCpred server, to motivate B cells. These peptides were bound to the largest number of alleles. Also they weren't toxigenic and allergenic epitopes that were investigated by Toxinpred and AllerTop servers. Designed chimeric protein was suitable in terms of physicochemical properties and stability.Conclusion : Epitopes vaccine design study showed that, predicted epitopes were with high efficiency and stability that can be used as a therapeutic peptide vaccine to be selected for prevention of SARS-CoV-۲ infection.