Investigation of Antimicrobial Effect of Ziziphus jujube on nosocomial bacterial infections

Background and Aim : The Incidence of drug resistance against chemical antimicrobial drugs has led to attention to the use of medicinal plants in the treatment of infections. The aim of this study was to evaluate the antimicrobial activity of extracts of Ziziphus jujube against pathogenic bacteria especially nosocomial infections.Methods : In this experimental study, aqueous and alcoholic extracts of, Ziziphus jujube were extracted. Inhibitory effects of plant extracts against Pathogenic bacteria (Staphylococcus saprophyticus, Escherichia coli, Salmonella typhimurium and Proteus mirabilis) and Pathogenic nosocomial (Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter and Pseudomonas aeruginosa) Through Disk diffusion methods, well diffusion method, and microdilution were investigated. Serial dilutions of the extracts were prepared in the range of ۵۰ to ۱۰۰۰ mg / ml to determine the minimum inhibitory concentration.Results : The aqueous extract of the plant showed higher inhibitory effects against microbial strains than the alcoholic extract. The two strains of Staphylococcus saprophyticus and Staphylococcus aureus showed greater sensitivity than extracts (aqueous, methanol, ethanol). Statistically, there was a significant difference in the minimum inhibitory concentration of aqueous extract growth compared to alcoholic extracts The aqueous extract on gram-positive bacteria of Staphylococcus saprophyticus and Staphylococcus aureus had a minimum inhibitory concentration of ۱۳۳ mg / ml and a minimum bacteriocidal concentration of ۲۰۰ mg / ml.Conclusion : The study found that Ziziphus jujube extract had significant effects on microorganisms, inhibitory zone of two gram positive bacteria including , Staphylococcus saprophyticus, ۵۷ mm in diameter, Staphylococcus aureus, ۵۵ mm in diameter, And the bacterium Pseudomonas aeruginosa showed the highest resistance between gram-negatives bacteria. The extracts also showed significant effects compared to the antibiotics as contorol. Although further research is needed in this regard, Ziziphus jujube extract can be suggested as a new antimicrobial agent in medicine resarch.

کلیدواژه ها:

Medicinal plant extract ، Pathogenic bacteria ، MIC Minimum Inhibitory Concentration ، Digestive and skin diseases