Use of Cinnamon and Garlic Essential Oils as an Alternative to Antibiotics in the Treatment of Aeromonasis in Fish

Background and Aim : In the aquaculture industry, one of the important problems is bacterial infectious diseases. Accordingly, farms fish of Kerman province was the aim of the present research and Aeromonas hydrophila isolation and identification of infection from coldwaterMethods : . Samples were from the skin and kidney of ۹۰ infected fish with signs of hemorrhagic septicemia in gill and skin, exophthalmia and dropsy, as well as ۳۶ water samples, were also taken from fish farms aseptically and subculture on TSA. From ۱۲۶ isolates of the fish and water samples, ۲۴ isolates of gram-negative bacteria were identified as Aeromonas at genus level by biochemical characteristics. After isolating the bacteria and PCR, different concentrations of cinnamon and garlic essential oil (۵۰, ۱۰۰, ۱۵۰ and ۲۰۰ ppm) were tested on the bacteria and the best antimicrobial concentrations of essential oils were determined.Results : The result showed ۵۰ ppm concentration essential oil of Garlic and ۱۰۰ ppm concentration essential oil of Cinnamon is the best of level bactericide. The combined effect of different treatments of essential oil and its different concentrations also caused a significant difference (P <۰.۰۵) in the growth of bacteria in the experimental culture medium.Conclusion : It can be concluded that the plant essential oils used in this study have antibacterial properties with varying degrees and effectiveness.

کلیدواژه ها:

Aeromonas hydrophila ، Garlic ، Cinnamon ، Antibacterial