Investigation of the Inhibitory Effect of the Proposed Chemical Composition on the Activity of Streptococcal Sortase A in Control of Dental Caries Invitro

Background and Aim : One of the most common childhood problems is tooth decay, and viridans group streptococci are the most common bacteria that cause the infection. The purpose of the study was to investigate the inhibitory effect of triclosan on the activity of streptococcal sortase A enzyme in controlling dental caries in vitro condition.Methods : This study was done on specimens isolated from teeth of pupils (n =۱۵۰ ) in Golestan province in ۲۰۱۹. After identifying Streptococcus viridans strains using specific tests, The SrtA gene was identified using polymerase chain reaction (PCR) with specific primers. Broth microdilution test was used according to CLSI M۱۰۰-S۲۵(۲۰۱۵) criteria to determine the minimum inhibitory concentration(MIC) of triclosan.Results : The frequency of S. viridans isolates containing Srt A was ۸۷ %. There was a significant difference between the frequency of tooth decay and presence of srt A (P<۰.۰۵). Determining MIC of triclosan showed that ۶۱% isolates of S.viridans tested were susceptible (MIC, ۱۶?g/ml) and ۳۹% (MIC, ۶۴?g/ml) of isolates were resistant to triclosan respectively. The concentration of triclosan that inhibited ۹۰% of isolates (MIC۹۰) was ۲۵۶ ?g/mL.Conclusion : The results demonstrated that there is a relationship between the presence of sortase A and dental caries caused by isolates and triclosan showed a good antibacterial effect in vitro condition.

کلیدواژه ها:

Streptococcus viridans ، Sortase A ، Triclosan ، Antibacterial effect