Antibacterial effects of extract of Pistacia khinjuk on Streptococcus pyogenes in laboratory condition

Background and Aim : Pistacia khinjuk (P. khinjuk) is a species of plants in the family Anacardiaceae and mostly native to Iran, Afghanistan, Egypt and India. Increasing antibiotic resistance and sensitivity reactions to chemical compounds are the main reasons that researchers are trying to find alternative antimicrobial herbal compounds. Extract of P. khinjuk is recently effective as an antimicrobial compound against some microorganisms. The purpose of this investigation is to determine the effects of the extract of P. khinjuk on Streptococcus pyogenes. St. pyogenes causes both invasive and noninvasive diseases such as Pharyngitis, Acute Rheumatic fever, Impetigo, etc.Methods : This research is performed in the laboratory by antibiogram testing method and measuring the inhibitory aura's diameter. In this way, first of all the three parts of P. khinjuk (outer membrane, inner membrane and seed) are separated and sterilized by UV-ray. Then three Erlenmeyer flasks containing ۲۰۰ ml of sterile distilled water and three Erlenmeyer flasks containing ۲۰۰ ml of ۷۵% ethanol are separated and ۳۰gr of the samples are added separately to the Erlenmeyer flasks. Subsequently, ۱۰۰۰ λ of the extract are diluted with DMSO and transferred to blank disks. Finally, the disks are transferred to the Blood agar culture medium containing St. pyogenes.Results : The results showed that among the six extracts, the ethanolic liquid extract of the inner membrane of P. khinjuk is the only extract that showed inhibitory aura after ۲۴h incubation. The aura's diameter is about ۹±۰/۵ mm.Conclusion : The ethanolic liquid extract of the inner membrane of P. khinjuk fruit on St. pyogenes in laboratory condition has a few inhibitory property. The development of herbal antibiotics is an alternative way to reduce consumption of chemical antibiotics.

کلیدواژه ها:

Pistacia khinjuk ، Streptococcus pyogenes ، antibiotic ، bacteria ، antibiogram