The anti-bacterial effect of Eucalyptus and Medlar extracts against Klebsiella pneumonia from Rasht hospitals

Background and Aim : Using plant based drugs against drug resistant bacterial infections is gaining special importance. The aim of this study was to evaluate the prevalence of extended spectrum beta lactamase producing clinical isolates of Klebsiella pneumonia and the antibacterial effects of aquatic and ethanol extracts of Eucalyptus and Medlar against these bacteria.Methods : In this study, a total of ۴۵ isolates of K. Pneumonia were collected from urinary tract infections. ESBL production was determined by the double disk diffusion and disk diffusion tested and specific primers PCR method was used to detect TEM and SHV genes. To investigate inhibitory effect of Eucalyptus and Medlar leaf extracts against ESBLs harboring ESBL isolates, well diffusion method and broth macro dilution method was used to determine the minimum inhibitory concentration and minimum bactericidal concentration.Results : ۲۶ Out of ۴۵ isolates, ۵۷ % phenotypically recognized as ESBL producing and based on the results of PCR, the prevalence of SHV genes among ESBLs-positive isolates was ۴۲% SHV and ۱۱% TEM positive isolates were detected. MIC of Eucalyptus extracts ranged between ۱۷/۵ - ۳۱mg/ml and Medlar extracts ranged between ۱۶۵-۲۵۰ mg/ml. MBC of Eucalyptus extracts ranged between ۶۰۰-۱۰۰۰mg/ml and Medlar extracts ranged between ۵۰۰-۱۰۰۰mg/ml.Conclusion : The obtained results showed Eucalyptus and Medlar extracts possess significant antibacterial activity against resistant bacteria. So these plant extracts may be used as an accomplishment in Klebsiella infection treatment, particularly in topical treatment of urinary tract infections.

کلیدواژه ها:

Klebsiella pneumonia ، ESBLS ، Eucalyptus ، Medlar ، TEM ، SHV