Determining the frequency of the presence of two fimH, mrkD genes in ESBL-positive Colebisella pneumoniae isolates in hospitalized patients

Background and Aim : Klebisella pneumoniae is a gram-negative bacterium that is involved in nosocomial infections, including respiratory, urinary, and blood infections. This bacterium has various pathogenic factors including fibrils, capsules, membrane transmitters, siderophores and LPS. FimH and mrkD are the sticky parts of the femur that play an important role in various infections. The aim of this study was to determine the frequency of two genes, fimH and mrkD, among the positive ESBL klebisella pneumoniae isolates in hospitalized patients in the cardiac hospital.Methods : In a descriptive, cross-sectional study, ۱۰۰ positive ESBL klebsiella pneumoniae isolates were selected. In this study, the antibiotic resistance pattern of these isolates was performed by diffusion disc method. After DNA extraction with the help of commercial kit, ESBL enzyme classes including SHV / TEM / CTX-M and frequency of FimH and mrkD genes were evaluated by PCR method.Results : : Among the studied isolates, the most common enzyme class SHV / TEM / CTX-M, ESBL was examined which had one, two or all three. The frequency of CTX gene was ۷۱.۱% and the frequency of SHV gene was ۴۷.۱%. The frequency of the TEM gene was ۶۴.۴%. The frequency of mrkD gene was ۸۲.۸% and the frequency of fimH gene was ۸۶.۶%.Conclusion : The mrkD and fimH genes are the most common coding genes for pathogenic factors in the positive strains of ESBL.

کلیدواژه ها:

Klebsiella pneumoniae ، ESBL ، hospitalized patients ، fimH ، mrkD