Investigation of the effect of liposome containing clove essential oil on Pseudomonas aeruginosa and Escherichia coli as nosocomial infections

Background and Aim : The aim of this study was to investigate the antibacterial effect of liposome containing clove essential oil. For this purpose, the lipid system containing clove essential oil has been synthesized for nosocomial infections Pseudomonas aeruginosa and Escherichia coli antibacterial methods.Methods : The type of study is laboratory research. The nanoparticle synthesis method is Mozaffari method. Particle characterization has been performed in terms of size and charge with DLS and morphology with the Atomic Force Microscope (AFM) and the amount of loading and release with the spectrophotometer. MIC tests were then performed to evaluate the performance of nanoparticles containing clove essential oil on Pseudomonas aeruginosa and Escherichia coli.Results : The average particle diameter was ۴۶ nm and its zeta potential was -۱۲.۷ mV. The loading rate in nanoparticles was ۶۳%, which was calculated by reading the absorption of light from the standard Trachyspermum Copticum curve. The minimum inhibitory concentration (MIC) of Pseudomonas aeruginosa and E.coli for nanoparticles was ۱۵.۶۲۵ and ۳۱.۲۵ mg / ml.Conclusion : Nanoparticles containing clove essential oil kill nosocomial infections Pseudomonas aeruginosa and Escherichia coli and can be used as antibacterial nano-systems.

کلیدواژه ها:

clove ، Antibacterial ، Pseudomonas aeruginosa ، Escherichia coli