Investigate the association of Phenotypic and Genotypic of Biofilm Formation in Acinetobacter baumannii

Background and Aim : Acinetobacter baumannii is an opportunistic pathogen that have persistence and survival ability in the hospital environment due to its multiple antibiotic resistance and ability to formation biofilms, and has been made as a serious problem health care worldwide.Methods : Seventy clinical isolates of the Acinetobacter baumannii were collected and identified using biochemical tests. Biofilm formation ability was performed by crystal violet staining. Isolates were tested for the presence of biofilm related genes (bap, epsA, blaPER-۱, pgaB) by PCR method.Results : The phenotypic results of biofilm formation showed that ۱۹ (۲۷.۱۴%), ۳۰ (۴۲.۸۵%), ۲۱ (۳۰.۰۶%) of the isolates had weak, moderate, and strong activities, respectively. The prevalence of pgaB, epsA, bap and blaPER-۱ genes were ۹۸,۶%, ۹۱.۹%, ۷۳.۲% and ۹۳.۲%, respectively. All isolates had at least one gene associated with biofilm and ۴۴.۲% of isolates carried ۴ biofilm related genes, simultaneously. Statistical analysis showed there was significant association between the ability to form biofilms and the presence of bap gene (P < ۰.۰۵).Conclusion : Our study revealed the high prevalence of biofilm related genes and the correlation between the frequency of bap gene with the of biofilm formation.Therefore, that the prevention and treatment measures are necessary the Acinetobacter baumannii strains in our country

کلیدواژه ها:

Acinetobacter baumannii ، biofilm formation ، biofilm related gene