Antibiotic resistance profiles of Pseudomonas aeruginosa strains isolated from urinary tract infections in Kerman, Iran

Background and Aim : Treatment of Pseudomonas infections is difficult right now due to the recent decreased in antibiotic sensitivity. So, our information on antibiotic resistance patterns should be upgraded for appropriate therapy. Accordingly, the aim of this research was to evaluate the antibiotic resistance profiles of Pseudomonas aeruginosa strains related to urinary tract infections.Methods : In this study, ۱۵ isolates of Pseudomonas aeruginosa were isolated from ۸۴ specimens isolated from patients with urinary tract infection in Kerman hospitals (Afzalipour and Shafa). Fifteen isolates were selected and disk diffusion susceptibility testing was performed for the antibiotics gentamicin, ciprofloxacin, imipenem, ceftazidime, tobramycin, cefepime, meropenem and cefotaxime and due to the higher sensitivity of the isolates to gentamicin Imipenem was determined by MIC micro agar dilution method.Results : Results showed sensitivity to Gentamicin ۱۱.۲۵ (۷۵%), ciprofloxacin ۹ (۶۰%), imipenem ۹.۷۵ (۶۵%), ceftazidime ۹.۷۵ (۶۵%), Cefepime ۹ (۶۰%), Tobramycin ۱۱.۲۵ (۷۵%) and cefotaxime ۰.۷۵ (۵%) and meropenem ۸.۲۵ (۵۵%). The highest antibiotic resistance of Cefotaxime (۵۰%) and the lowest antibiotic resistance (۲۵%) were estimated in Gentamicin, Cefepime and Tobramycin. Two-bacterial hydrophobicity test was used to select bacteria for biofilm testing with HI below ۳۰% (IAUK۱۱۴۳ and IAUK۱۱۴۷) and above ۷۰%( IAUK۱۱۴۲ and IAUK۱۱۳۹). Strong hydrophobicity was selected to evaluate biofilm formation. The rate of biofilm formation in the plastic tube was higher than glass tube under both constant and conditions. In the case of isolates with strong hydrophobicity, the absorbance in the plastic tube is higher than in shaking condition and in the glass tube, the absorbance is higher in the static condition.Conclusion : So, these data indicate that both antibiotic resistance and biofilm formation of most isolates are responsible for complicated therapy and physicians must receive the recent information about these traits of clinical strains of P.aeruginosa.

کلیدواژه ها:

Pseudomonas aeruginosa ، MIC ، biofilm ، Antibiotic resistance