Detection of blaIMP۴, bla CTX-M, tetA and aadB genes among Acinetobacterbaumanii clinical strains isolated from Imam Khomeini, Bahman, Bu-Ali and Momenin hospitals in Tehran by PCR

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 200

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شناسه ملی سند علمی:

MEDISM21_118

تاریخ نمایه سازی: 23 مرداد 1400

چکیده مقاله:

Background and Aim : Acinetobacterbaumannii is an opportunistic bacterial pathogen responsible fora wide range of hospital-acquired infections. These bacteria take a variety of factors for resistance to different antibiotics, including resistance to β-lactams, aminoglycosides, and tetracyclines. The aims of this study were to determine the antimicrobial susceptibility pattern and prevalence of blaIMP۴, bla CTX-M, tetA and aadBgenes in A.baumannii strains obtained from Imam Khomeini, Bahman, Bu-Ali anda Amir Al-Momenin hospitals.Methods : In this cross-sectional study, ۱۰۰ clinical Acinetobacterbaumannii isolates were collected from various hospitals in Tehran. After identification of Acinetobacterbaumannii isolates by biochemical tests, the antibiotic susceptibility test (Kirby-Bauer method) was done according to CLSI advice against ۹ antibiotics. Finally, the blaIMP۴, bla CTX-M, tetA and aadB genes were determined among the antibiotic resistant isolates using PCR.Results : The disc diffusion results showed resistance rates of ۹۰% for ciprofloxacin, ۳۲% ceftazidime, ۲۵% imipenem, ۳۶% gentamicin, ۳۴% streptomycin, ۲۸% piperacillin, ۵% polymyxin B and ۶۳% tetracyclin. All isolates were susceptible to colistin. PCR results forblaIMP۴, bla CTX-M, tetA and aadB genes were detected in ۶۳%, ۶۲%, ۷۶% and ۷۱% of resistant isolates respectively.Conclusion : This study detected clinical A. baumannii isolates harboring antibiotic resistance genes. Identification of antibiotic resistance patterns in A. baumannii and investigation of molecular epidemiology are critical to control the rapid spread of antimicrobial resistant strains.

نویسندگان

Farisa Majidianfar

Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Setareh Haghighat

Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Farshad Hashemian

Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Bahareh Norouzi

Department of Biology, Science and Research Branch, Islamic Azad University,Daneshgah Blvd, Simon Bulivar Blvd, Tehran, Iran