DETECTION OF MECA GENE AMONG METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM BLOOD INFECTION IN AN EDUCATIONAL AND THERAPEUTIC HOSPITAL OF SARAB IRAN

Background and Aim : Methicillin-resistant Staphylococcus aureus (MRSA) is one of the clinically significant pathogens that cause various nosocomial and community acquired infections in human. Nowadays, high prevalence of MRSA among bloodstream infections is concerning and its resistance to different types of common antibiotics has limited treatment options. MecA gene that encodes a protein called penicillin binding protein ۲a (PBP۲a) is responsible for resistance to methicillin in staphylococci. The present study aimed to determine the prevalence of MRSA and antibiotic susceptibility patterns of the isolates at Sarab Imam Khomeini Educational and Medical CenterMethods : In a period of one year (Aug. ۲۰۱۸ to Aug. ۲۰۱۹) S. aureus strains were isolated by conventional culture techniques and standard biochemical tests from patients at Sarab Imam Khomeini Educational and Medical Center. Susceptibility of the isolates against several antibiotics was evaluated by disk diffusion method. Methicillin resistant isolates were subjected to PCR analyze for the presence of mecA gene.Results : Out of ۱۱۰ blood samples, ۲۲% of isolates were identified as S.aureus. Among S.aureus strains, ۳۰% were resistant to cefoxitin disk and ۶ strains were positive for mecA gene, according to the guidelines of Clinical and Laboratory Standards Institute (CLSI). According to results, polymerase chain reaction (PCR) assay is a useful and reliable way for rapid detection of MRSA strains and plays a big role in prognosis of blood stream infectionsConclusion : Findings in this study indicate the high prevalence of MRSA among the isolates at Sarab hospital

کلیدواژه ها:

Bloodstreem infection ، S. aureus ، MRSA ، mecA