EVALUATION OF ANTIBIOTIC RESISTANCE PATTERN AND EFFICACY OF MODIFIED HODGE TEST FOR DETECTION OF CARBAPENEM-RESISTANT KLEBSIELLA PNEUMONIAE STRAINS ISOLATED FROM CLINICAL SAMPLES

Background and Aim : Klebsiella pneumoniae is a gram-negative opportunistic pathogen belonging to the Enterobacteriaceae family and can cause several infections with high rates of morbidity and mortality in human. Nowadayes, the emergence of carbapenem-resistant Klebsiella pneumoniae strains has become a big threat for public health. The aim of this study was to evaluate the prevalence of carbapenemase producing Klebsiella pneumoniae strains among various clinical specimens at Imam Khomeini Educational and Treatment Center of Sarab.Methods : In this study, ۱۳۰ different clinical samples were collected from patients included ۷۳ (۵۶%) females and ۵۷ (۴۴%) males at Imam Khomeini Educational and Treatment Center of Sarab. K. pneumoniae strains were isolated by different culture techniques and standard biochemical tests. Standard disk-diffusion method was used for evaluate antibiotic resistance pattern and The Modified Hodge Test was performed for detection of KPC-producing strains based on the instructions of Clinical Laboratory Standards Institute (CLSI).Results : The highest and the lowest rate of resistance in isolated strains were observed for Piperacillin (۷۸%) and Ertapenem (۴۴%) respectively. The Modified Hodge Test was positive for ۷۳ (۵۶%) isolates that the highest rate of resistance was observed for piperacillin (۸۸%) and cefotaxime (۷۹%).Conclusion : The results of this study represent increasing prevalence of carbapenemase producing Klebsiella pneumoniae isolates. Therefore, it is necessary to modify the antibiotic consumption pattern for effective therapy

کلیدواژه ها:

Klebsiella pneumoniae ، Carbapenemase ، Modified Hodge Test