Does selenium nanoparticles prevent the adherence of Candida parapsilosis and Enterococcus faecalis to urinary catheters?

Background and Aim : Catheter related urinary tract infections continue to be a major cause of morbidity in hospitalized patients. The prominent role of biofilm in the pathogenesis of these infections has been suggested in the past few years. The aim of this study was to evaluate in vitro effect of selenium nanoparticles (SeNPs) on the formation of Candida species and Enterococcus species biofilms on urinary catheters.Methods : In order to prepare yeast and bacterial suspensions (۰.۵ McFarland), ۰.۵ cm pieces from catheter were incubated at ۳۷ C in tryptic soy broth medium. Then, the pieces were washed with PBS to remove nonadherent microorganisms. After biofilm formation, the effect of SeNPs at concentrations of ۶۴ to ۰.۱۲۵ μg/mL were investigated using broth microdilution method (CLSI, M۲۷-S۴).Results : Minimum inhibitory concentration (MIC) was determined to evaluate the anti-adherent effect of SeNPs on microorganisms. Totally effect of SeNPs on E. faecium strains adherence was lower than C. parapsilosis strains, significantly (p < ۰.۰۱).Conclusion : The pathogenicity of multiple Candida species and Enterococcus species is mainly associated with their ability to form biofilms; so that, early steps in the establishment of infections are adherence and biofilm formation. Furthermore, we concluded that SeNPs has a considerable anti-adherent activity against C. parapsilosis by decreasing MIC. This study suggests that coating catheter with SeNPs could be effective to prevent catheter related urinary tract infection through inhibition of microbial adherence.

کلیدواژه ها:

Candida parapsilosis ، Enterococcus faecalis ، Selenium nanoparticles ، Adherence