Associations of a NLRP۳ rs۱۰۷۵۴۵۵۸ polymorphism with Helicobacter pylori-infected patients with gastritis and peptic ulcer disease

Background and Aim : Diseases caused by Helicobacter pylori infection, including gastritis and gastric ulcer, can be harmful to epithelial cells in gastric mucosa. Furthermore, pro-inflammatory cytokines can affect the severity of some diseases caused by H. pylori infection. NLRP۳ inflammasome can detect H. pylori and trigger caspase-۱ activation and thus IL-۱β release in the stomach. Since genetic variations such as polymorphisms might be responsible for the expression of genes, the present research aimed to explore the effect of NLRP۳ rs۱۰۷۵۴۵۵۸ polymorphism in pathogenesis of H. pylori infection.Methods : genotyping was performed on ۴۶۴ Iranian patients (۳۰۰ patients infected with H. pylori and ۱۶۴ patients uninfected with H. pylori) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Cytokine mRNA and protein expression were evaluated in patients infected with different genotypes of NLRP۳ rs۱۰۷۵۴۵۵۸ using real time-PCR and western blotting, respectively.Results : The NLRP۳ rs۱۰۷۵۴۵۵۸ gene polymorphism was not associated with H. pylori infection or diseases caused by this bacterium. It was found that the IL-۱β level in infected patients with gastritis was more than in the uninfected individuals. Moreover, no relation was found between these single nucleotide polymorphisms (SNP) and cytokine expression in H. pylori infected subjects. The findings of this study show that NLRP۳ rs۱۰۷۵۴۵۵۸ polymorphism may be related to the severity of acute inflammation in these patients.Conclusion : Our findings show that NLRP۳ rs۱۰۷۵۴۵۵۸ polymorphism may be involved in the severity of some diseases caused by H. pylori infection.

کلیدواژه ها:

Helicobacter pylori ، gastritis ، peptic ulcer disease ، polymorphism ، NLRP۳ rs۱۰۷۵۴۵۵۸