Bacteriological examination and diagnosis of root canals associated with dental periapical abscesses

Background and Aim : The aim of this study was to identify microorganisms from root canals with periapical abscesses and to ascertain the susceptibility of Peptostreptococcus prevotii and Fusobacterium necrophorum to antimicrobials. Study design Thirty root canals were microbiologically sampled by using sterile paper points. The concomitant microorganisms were identified through the use of established methods. The susceptibility of P prevotii and F necrophorum to antimicrobials was evaluated by using the E test method.Methods : Culture procedures have traditionally been used in the assessment of the microbiota associated with vari- ous infectious diseases, including infections of endodontic origin. The culturing techniques have a reasonable degree of agreement in terms of the identification of oral microorganisms compared with that of the “checkerboard” DNA-DNA hybridization method. The major advantage of the culture procedure is its ability to enable the detection of unexpectedly viable cells (molecular procedures enable the detection of only target microbial species ). Many molecular techniques assist in the identification of cultivable microorganisms.Results : A total of ۱۱۷ different bacterial strains were recovered, including ۷۵ strict anaerobes or microphilic species. The most frequently isolated strict anaerobes were P prevotii, Peptostreptococcus micros, and F necrophorum. Facultative bacteria such as Gemella morbillorum and Streptococcus mitis were also found, albeit less frequently.Conclusion : Gram-positive anaerobic bacteria predominate in the mixed microbiota of root canals with periapical abscesses. Moreover, P prevotii and F necrophorum are susceptible to the tested antibiotics.

کلیدواژه ها:

Bacteriological ، periapical abscesses ، root canals