Evaluation of incidence of Ch.trachomatis and N.gonorrhoeae in women with endoservitis by PCR method

Background and Aim : Introduction and Objectives: Neisseria gonorrhoeae and Chlamydia trachomatis are the two most common bacterial sexually transmitted infections that manifest primarily as urethritis in males and endocervicitis in females. Recently molecular amplification assays like Polymerase Chain Reaction (PCR) and Ligase Chain Reaction (LCR) have been found to be highly sensitive and specific methods for detection of N. gonorrhoeae and C. trachonmatis not only in urethral and cervical specimens but also in urine. In women, the cervix is the most common site of gonorrhea, resulting in endocervicitis and urethritis, which can be complicated by pelvic inflammatory disease (PID). The study aimed to assess the prevalence of N.gonorrhoeae and C.trachomatis infections rate in women with endoservisit.Methods : Materials and Methods: In this study endocervical smears and urine samples collected from ۱۵۰ women with genitourinary infections were analyzed after DNA extraction by using PCR technique and special primers (KL۱,KL۲ and SL۵۹,SL۶۷) to determine the C.trochomatis and N.Gonorrhoea.Results : RESULTS: In our study prevalence of Chlamydia infection in urine samples was ۸.۱% and in Endoservical swab (ESS) was ۹.۸% and prevalence of N.gonorrhea was ۱% in urine samples and ۰.۷% in ESS samples. Coinfection prevalence was ۰.۴% with analyzing urine samples and it was ۰.۶ in ESS samples.Conclusion : In conclution in sexually transmitted diseases two most important pathogens, C. N .gonorrhoea which are difficult to diagnosis with conventional culture me more comfortable for the patients PCR-based methods are seen to be p: samples of cervical swab which have smilar sensitivity, although these easy, inexpensive, and highly sensitive.

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Key worlds: C.trochomatis ، N.gonorrhoea ، Endocervitis ، PCR