Background and Aim : Matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases that play important roles in the degradation of extracellular matrix (ECM) and various inflammatory diseases. Also, they affect cancer development processes, such as apoptosis, cell proliferation, and immune system. Therefore, this study aimed to investigate the induction of MMP۲, -۷, and -۹ by different H. pylori strains in the MKN-۴۵ cell line. We also determined correlation of MMPs mRNA expression levels with virulence factors of the strains and type of disease.Methods : Nineteen H. pylori strains which were isolated from biopsy specimens of dyspeptic patients with different histopathological status were selected. Fresh colonies of H. pylori were co-cultured with MKN-۴۵ cells at a multiplicity of infection of ۱۰۰ for ۱ and ۶ hours. Then, the expression of MMP-۲, -۷, and -۹ messenger RNA was measured by a quantitative real-time PCR method. The presence of virulence genes, including cagA, vacA (s/m), babA (A۲), and sabA was performed using PCR analysis as wellResults : Histopathological status consisted of ۱۰.۵% intestinal metaplasia (IM), ۲۶.۳% chronic gastritis (CG), and ۶۳.۲% severe active gastritis (SAG) that ۶۸.۴۱% and ۳۱.۵۹% of them were non-ulcer diseases (NUD) and peptic ulcer disease (PUD), respectively. Status of cagA, vacA s۱, vacA s۲, vacA m۱, vacA m۲, iceA۱, iceA۲, iceA۱+A۲, babA۲ and sabA genes in isolates were ۷۸.۹%, ۷۳.۷%, ۲۶.۳%, ۵.۳%, ۹۴.۷%, ۳۶.۸%, ۵.۳%, ۴۷.۴%, ۱۰۰%, and ۹۴.۷%, respectively. Upregulation of MMP۲, MMP۷ and MMP۹ genes were detected at ranges of ۱۵.۷۳%, ۵.۲۶%, ۱۵.۷۸% (۱ hour co-culturing), ۲۱.۰۵%, ۲۶.۳۱%, ۱۵.۷۸% (۶ hour co-culturing) respectively. Further, downregulation of MMP۲, MMP۷and MMP۹ genes were respectively detected at ranges of ۳۶.۸۴%, ۱۰.۵۲%, ۱۵.۷۸% (۱ hour co-culturing), ۳۶.۸۴%, ۰%, ۱۰.۵۲% (۶ hour co-culturing). Considering, the results indicated that the infection of MKN-۴۵ cells with H. pylori did not leadto an increase or decrease in levels of MMPs messenger RNA significantly. Furthermore, the induction of MMPs wasn’t correlated with virulence factors of the strains and type of disease.Conclusion : Our finding showed a variation in the induction of MMP۲, -۷, and -۹ gene expression in MKN-۴۵ cell by different strains of H. Pylori. As a result, further studies are needed to evaluate the possible role of H. pylori on the induction of MMP۲, -۷, and -۹ in human gastric.