Comparison of antimicrobial and wound healing properties of mouse adipose tissue- and bone marrow-derived mesenchymal stem cells in fibrin scaffold on burn wound infection triggered by Pseudomonas aeruginosa

Background and Aim : Burn is a leading cause of injury and the most devastating forms of trauma. Pseudomonas aeruginosa has become a common cause of burn wound infection. Recently, a new method using mesenchymal stem cells has been developed for the recovery of burn wounds. Fibrin hydrogel scaffold has been addressed to release mesenchymal stem cells.Methods : The stem cells were extracted from mouse adipose and bone marrow tissues and then were confirmed by the surface markers using flow cytometry analysis. The possibility of differentiation of the stem cells into bone marrow and adipose were also checked. The extracted stem cells were encapsulated in the fibrin scaffold. Fibrin-encapsulated mesenchymal stem cells were used to dress wound following induction of burn infection in mice. The microbial load was measured by the colony count method in sacrificial mice.Results : In vivo studies showed that the adipose-derived mesenchymal stem cells had strong antimicrobial characteristics on P. aeuroginosa-induced burn wound, while the bone marrow-derived mesenchymal stem cells improved wound regeneration/healingConclusion : We showed that the fibrin-encapsulated mesenchymal stem cells could regenerate lost wound tissue and significantly reduced the load of bacteria.

کلیدواژه ها:

Burn wound infection ، Fibrin scaffold ، Mesenchymal stem cells ، Pseudomonas aeruginosa