Study of oral microbiome in MS patient compared with healthy people

Background and Aim : Word " microbiome" was used by Lederberg for definition of commensal, symbiotic ecologic population and pathogenic microorganism. Microorganisms in oral cavity are called oral microflora, Researchers believe that oral microbiome is important in systemic diseases. Oral cavity is suitable for microbiome because of its temperature (almost ۳۷degree), PH (۷.۵) and humidity. Oral microorganisms include different genera of bacterium. Interaction between host and microorganisms is important in nervous diseases such as Autism, Alzheimer, Parkinson and Multiple Sclerosis (MS) . Recently researchers studied on bacterial infection as one of environmental factors of MS. Importance of bacteria is their role in increase of Th۱۷. Th۱۷ increase production of IL-۲۱, IL-۱۷, IL -۲۲ that are inflammatory cytokines. In this study for first time is studied oral microbiome in MS patients and is compared with healthy people.Methods : Sampling was performed in MS patients center of Kermanshah University of Medical Sciences during October to March ۲۰۱۹. ۳۰ samples were recruited from MS patients and ۳۰ samples were obtained from healthy people. From each person was taken ۲ swab samples from on and side of mouth that one swab was sunk into PBS and another one into Thioglycollate broth for increase turbidity in aerobic and an aerobic condition. Brucella blood agar supplemented hemin and vitamin K was used for this studing, After pure cultures were obtained , identification of bacteria was performed by Phenotypic tests including gram staining, catalase, nitrate, Aerotolerance test, Antibiogram test, bile esculin agar test.Results : In this study Staphylococcus, Streptococcus, Enterococcus, Peptostreptococcus, Actinomyces, Lactobacillus, Fusobacterium, Bacteroides, Porphyromonas, Prevotella, Veillonella, Propionibacterium and Bifidobacterium genera were isolated that in all cases the number of bacteria were more in patient group except Peptostreptococcus and Lactobacillus and Prevotella and Propionibacterium genera were found just in patient samples.Conclusion : It seems oral microbiome composition is important in MS disease development as the effect of gut microbiome in MS disease was confirmed in previous studies. High number of useful genera such as Lactobacillus in healthy group shows that take a meal containing useful bacteria can help in control of inflammatory conditions and as a result control of MS disease development.

کلیدواژه ها:

Microbiome ، Multiple Sclerosis and Bacteria