Molecular analysis of Methicillin-Resistant Staphylococcus aureus isolated from pemphigus patients

Background and Aim : Pemphigus is an autoimmune bullous disease that affects the skin and mucosas. Colonization of these skin wounds with MRSA can delay the healing process of the wound and increase the rate of infection and mortality in these patients. We therefore decided to conduct this study to determine the SCCmec and dru types, toxins, MSCRAMM and biofilm genes of MRSA isolated from Pemphigus patients.Methods : In total, ۱۱۸ S. aureus isolates were collected from wound infection of pemphigus patients. In order to detect MRSA isolates, the mecA gene was amplified through the polymerase chain reaction (PCR) method. Multiplex-polymerase chain reaction (MPCR) assay was performed for the characterization of the staphylococcal cassette chromosome mec (SCCmec). The dru region was sequenced and thereby, dru types and dru repeats were identified. A similarity matrix was used to create minimum spanning tree (MST). All MRSA isolates were assayed for the presence of the sea, seb, sec, tst, eta, pvl, hla, hlb, MSCRAMMs and ica genes by PCR.Results : Our study results illustrated that the prevalence of MRSA among S.aureus isolated from patients with pemphigus was ۴۳.۲% . Thirty-four (۳۵.۳%) of MRSA strains were SCCmec type II and Eighteen (۳۵.۳%) of MRSA strains were SCCmec type III. The successful dru typing of isolates revealed seven different dru types. There was a new dru type, namely dt۹ca. the prevalence rates of the hla،sea وsec genes among MRSA isolates were ۵۴.۹%, ۲۷.۴% and ۱.۹%, respectively. The hlb, seb, eta and pvl genes were not detected in any of the MRSA isolates. Our results revealed that ۱ isolate from pemphigus wound infection expressed the tst gene. The prevalence rate of MSCRAMM genes such as fib, eno, ebpS, were ۲۱.۵%, ۳۱.۳% and ۹.۸%, respectively. The fnbB, fnbA and cna genes were not detected in any of the MRSA isolates. Moreover, ۳.۹% and ۵.۸% of the isolates harbored the icaA and icaD genes respectively.Conclusion : This study shows the high prevalence of toxin genes such as hla, sea among MRSA strains with SCCmec II and III isolated from Pemphigus wounds in a dermatology centre in Tehran, Iran.

کلیدواژه ها:

MRSA ، SCCmec type ، MSCRAMMs ، Toxin genes ، Pemphigus