The Study Effects on Aqueous Extract of Ammi visnaga on the Pseudomonas aeruginosa Exo A and Exo S Genes

Background and Aim : Abstract Background: Pseudomonas aeruginosa is one of the important factors in many secondary diseases and infections including burns. The aim of this study was to investigate the effect of Ammi visnaga extract on the expression aeruginosa pathogenic genes. Materials and Methods: In this experimental study. Antimicrobial effects of Soxhlet extract using microdilution technique in sequential dilutions. Also, expression of exotoxin A and S genes as pathogens of Pseudomonas aeruginosa was evaluated by Real Time PCR method. Results: The results showed that expression of Exotoxin A and S genes of Pseudomonas was significantly (P <۰.۰۰۱) lower than the expression level of reference gene (۱۶s). Also,it had significant effect on inhibition of bacterial growth at different concentrations and the lowest inhibitory concentration was observed at ۵۰۰. Conclusion:The extract of ammi visnaga can decrease the expression of Exotoxin A and S genes of Pseudomonas aeruginosa and consequently decrease the number of bacteria.Methods : ۲. Methodology ۲-۱- preparation of plant ۲-۲-Bacterial strains and storage methods ۲-۳-Cultivation of Pseudomonas aeruginosa bacterium for the production of exotoxins ۲-۴-Determining the minimum MIC growth concentration of toothpaste extract ۲-۵- Investigating gene expression, RNA extraction, primer design and PCR reactionResults : ۳. Results ۳-۱-MIC and MBC test results and well, The lowest concentration in which no turbidity was observed was considered as the MIC, which was ۵۰۰. The MBC test results and MIC test wells are also presented. ۳-۲-Product Replication Curve shows: at the end of the reaction, a standard linear reaction curve based on Ct and logarithm of DNA concentration was drawn to confirm the proliferation of the PCR product. ۳-۳-Real-Time PCR results shows the effect of toothpaste extract on the expression of exoA and exoS genes of Pseudomonas aeruginosa.Conclusion : ۴- Discussion and conclusion The results showed that the plant extract reduced the expression of these genes, which are pathogenic bacteria. Also, the results of MIC test showed that the lowest bactericidal concentration of toothpaste extract is ۵۰۰. There is very little research done in this field, so that the discussion and comparison of the present study was difficult. However, some research in this area has yielded close and similar results, which we will examine.

کلیدواژه ها:

Ammi visnaga ، Pseudomonas aeruginosa ، Exotoxin A ، Exotoxin S