Isolation and In Vitro evaluation specific bacteriophage against methicillin-resistant Staphylococcus aureus and of its antibacterial effects compared to methicillin assay
محل انتشار: بیست و یکمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 217
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شناسه ملی سند علمی:
MEDISM21_006
تاریخ نمایه سازی: 23 مرداد 1400
چکیده مقاله:
Background and Aim : Staphylococcus aureus is an important causative agent of infections in various hospital wards. Today, antibiotic-resistant strains, especially methicillin-resistant Staphylococcus aureus (MRSA), are spreading rapidly, causing many problems in infection control and treatment. Bacteriophage (phage) is a group of viruses that can infect their host cells and multiply inside them, eventually causing cell lysis. Phage cannot infect eukaryotic cells and therefore can be used in infection therapy. The present study aims to evaluate the antibacterial and lytic activities of isolated bacteriophage against MRSA.Methods : The standard strain of MRSA (ATCC ۲۵۹۲۳) was obtained from the Department of Bacteriology, Pasteur Institute of Iran. The specific bacteriophage was isolated from hospital sewage using Soft agar. The morphology of isolated bacteriophages was evaluated using electron microscopy (TEM). The susceptibility of MRSA to the isolated bacteriophage was assessed by the Spot test. To obtain the appropriate titer for bactericidal results, different concentrations of bacteriophage were evaluated against MRSA. The binding and pathogenicity of MRSA on HEP-۲( Human epithelial type ۲) cell lines were evaluated.Results : The isolated bacteriophage was specific for S. aureus and had no lytic activity against other pathogenic bacteria. The binding and pathogenicity of MRSA to HEP-۲ cell line was evaluated, and as expected, the lytic activity of specific bacteriophage was observed following inoculation.Conclusion : Given the increased antibiotic resistance rate among S. aureus strains and growing concerns about the treatment of infections associated with these strains, this bacteriophage may be used as an effective therapeutic agent as well as a preventive measure in hospital environments.
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نویسندگان
MohammadReza EsmaeilZadeh
M.Sc., Department of Food Microbiology, Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Iran