Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population
محل انتشار: فصلنامه طب دامی ایران، دوره: 15، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 170
فایل این مقاله در 13 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJVM-15-2_002
تاریخ نمایه سازی: 12 مرداد 1400
چکیده مقاله:
BACKGROUND: Infectious bursal disease (IBD) is a highly contagious immunosuppressive disease of young chickens caused by infectious bursal disease virus (IBDV). IBDV consists of a two-segmented double-stranded RNA genome which can easily undergo genomic recombination or reassortment during mixed infections. OBJECTIVES: The objectives of this study were aimed to characterize a previously identified Iranian IBDV strain (JRMP۲۹IR) in specific-pathogen-free (SPF) chickens, evaluate the presence of a mixed and/or reassortant virus popula-tion in this strain, and examine the frequency of genomic recombination and reassortment in publicly available IBDV genomes through bioinformatics study. METHODS: The SPF chickens were challenged with the JRMP۲۹IR strain via oral and intraocular routes. Bursal tissues were extracted for histopathological evaluation and reverse transcription-polymerase chain reaction (RT-PCR) followed by Sanger sequencing. Putative recombinations and reassortments were evaluated using the Recombination Detection Program ۵. RESULTS: Through genomic sequencing of the viruses from the bursas of infected chickens, the JRMP۲۹IR strain was found which contained viruses from the classic, variant and very virulent IBDV genotypes. Through bioinformatics, nu-merous putative recombination and reassortment events were identified that naturally occur throughout the IBDV genome. CONCLUSIONS: Parental JRMP۲۹IR appears to be derived from a flock undergoing a mixed IBDV infection. High frequency of recombination and reassortment among IBDVs suggests that these events are evolutionarily beneficial for the virus
کلیدواژه ها:
نویسندگان
Amir Ghorbani
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Seyed Mostafa Peighambari
Department of Avian Diseases Faculty of Veterinary Medicine University of Tehran Tehran, Iran
Jamshid Razmyar
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :