A Comparative Study of Extraction Methods and Components Identification of Teucrium Chamaedrys and Investigation of Their Antibacterial Effect

Ethnopharmacological relevance: Teucrium Chamaedryswas historically used as a medicinal herb for the treatment of gout and sometimes as a component of Venice treacle. Aim of the study: Our aim was identification and extraction of components of Teucrium Chamaedrys using hydrodistillation as a usual method, microwave-assisted, and headspace solid-phase microextraction as two new methods and comparing their results. Chromatography/mass spectrometry (GC/MS) was used to investigate the estimated compounds. Finally, we study their antibacterial effect. Results: ۱۷ compounds were identified accounting for a total of ۸۵.۳۵% of the total essential oil obtained from the hydrodistillation method. In addition, ۱۵ compounds were found accounting for ۷۹.۲۶% of the total essential oil, obtained from the solid phase microextraction. In the microwave method, the essential oil obtained did not have the required quantity and quality to identify. Conclusion: By comparing the above results, it is concluded that more compounds can be isolated and identified through hydrodistillation.The results showed that the main composition of this plant is caryophyllene in different ways and a total of different forms of this composition in both methods showed that a higher percentage of these compounds is obtained in the steam distillation process, and factors such as plant drying and extraction method will have a significant effect on the extraction percentage. The antibacterial effect against gram-positive bacteria of the essential oil extracted by hydrodistillation and solid-phase microextraction methods was proved.

کلیدواژه ها:

Extraction ، Teucrium Chamaedrys ، Antibacterial Effect ، Hydrodistillation ، microwave-assisted ، Headspace Solid-Phase Microextraction