Fabrication, characterization, and experimental analysis of a novel Gelatin-based nanocomposite scaffold with bone substitute applications

In this study, a novel nano-composite bone scaffold composed of Gelatin (Gel) and Polypyrrole (PPY) biopolymers and Akermanite (AK) and Magnetite bioceramics is introduced. So, a ۱۰ w/v % Gelatin solution was obtained, and other materials were added to such solution gradually and finally, the suspension was frozen and freeze-dried for ۲۴h. Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction analysis (XRD) were implemented to identify thescaffold's organic and crystalline phases and as a way for proving the existence of named materials within the composite scaffold. According to the existence of the result of such materials proved. Afterward, the scaffolds' immerging in the Phosphate Buffer Saline (PBS) solution was done, and swelling of such scaffolds within such solution was studied. According to the results, the highest incline in the swelling diagram happened in the first ۷ h of such test, and after ۱۲۰ h, the highest value of the PBS solution absorption was seen with a value of ۱۰۱۲%. SEM images were taken. The morphology of such scaffolds in microscale was studied, and by analyzing such images in Image-j software, a ۶۸.۳% porosity volume fraction and a mean pore cell size of ۱۷۴.۰۵ 𝜇m were measured. The porosity volume fraction was in the range of the cancellous bone porosity volume fraction. Finally, the MTT assay was conducted, andfibroblast cells were cultured. According to the results, after three days, ۱۰۰% cell viability was seen. Such scaffolds are good candidates for in-vivo applicationhaving no toxic or inflectional effects according to the in-vitro non-toxicity

کلیدواژه ها:

Bio-polymer ، Bio-ceramic ، Bone substitutes ، Nano- Nano-composite ، Scaffold ، Porous structure.