Verbascoside alleviates renal fibrosis in unilateral ureteral obstruction rats by inhibiting macrophage infiltration

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 145

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شناسه ملی سند علمی:

JR_IJBMS-24-6_006

تاریخ نمایه سازی: 17 خرداد 1400

چکیده مقاله:

Objective(s): To explore the effect of verbascoside on renal fibrosis in unilateral ureteral obstruction (UUO) rats.Materials and Methods: Twenty Sprague-Dawley rats were randomly distributed into sham-operated, UUO, and UUO+Verbascoside groups. After two weeks of rat model construction, urine and blood samples were collected for biochemical analysis while kidney tissues were harvested for hematoxylin and eosin (H&E), Masson’s Trichrome, and immunohistochemistry staining. Pearson coefficient was used to analyze the correlation between the two proteins. Results: Verbascoside improved UUO-induced renal dysfunction as detected by decreased serum creatinine, urea nitrogen, and urinary protein excretion rate. In UUO rats, H&E staining result revealed increased total nucleated cell number, and Masson’s Trichrome staining results showed tubular interstitial fibrosis with the deposition of collagen fibrils. Besides, expressions of fibrosis-related proteins including collagen type I (COL-I), α-smooth muscle actin (a-SMA), and tissue inhibitor of metalloproteinase ۲ (TIMP۲) expressed higher in the UUO group. Moreover, macrophage infiltration-related factors such as CD۶۸+, F۴/۸۰+ cells, and suppressor of cytokine signaling-۳ (SOCS۳) were significantly higher in the UUO group than in sham-operated rats. However, after administration with verbascoside, the accumulation of collagen fibrils and total nucleated cell numbers were mitigated. Likewise, macrophage infiltration was extenuated and fibrosis-related proteins were down-regulated in the UUO+Verbascoside rats. Correlation analysis indicated that macrophage infiltration-related markers were related to fibrosis-related factors.Conclusion: Verbascoside could alleviate renal fibrosis in UUO rats probably through ameliorating macrophage infiltration.

نویسندگان

Guihua Zhang

Guizhou University School of Medicine, Guizhou University, Gui Yang, Gui Zhou, China

Fuxun Yu

The NHC Key Laboratory of Pulmonary Immunological Disease, Guizhou Provincial People’s Hospital, Gui Yang, Gui Zhou, China

Rong Dong

Guizhou University School of Medicine, Guizhou University, Gui Yang, Gui Zhou, China

Jiali Yu

Guizhou University School of Medicine, Guizhou University, Gui Yang, Gui Zhou, China

Meng Luo

Guizhou University School of Medicine, Guizhou University, Gui Yang, Gui Zhou, China

Yan Zha

Guizhou University School of Medicine, Guizhou University, Gui Yang, Gui Zhou, China

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  • Zhang ZH, He JQ, Qin WW, Zhao YY, Tan NH. ...
  • Cai K, Chai L, Luo Q, Dai Z, Wu L, ...
  • Martínez-Klimova E, Aparicio-Trejo OE, Tapia E, Pedraza-Chaverri J. Unilateral ureteral ...
  • You YK, Luo Q, Wu WF, Zhang JJ, Zhu HJ, ...
  • Zeng G, Mai Z, Xia S, Wang Z, Zhang K, ...
  • Wu J, Yang Z, Wei J, Zeng C, Wang Y, ...
  • Yang C, Wang H, Zhao X, Matsushita K, Coresh J, ...
  • Stevens S. Obstructive Kidney Disease. Nurs Clin North Am ۲۰۱۸; ...
  • Waasdorp M, Rooij DM, Florquin S, Duitman J, Spek CA. ...
  • Zhao J, Meng M, Zhang J, Li L, Zhu X, ...
  • Xianyuan L, Wei Z, Yaqian D, Dan Z, Xueli T, ...
  • Yang L, Yuan H, Yu Y, Yu N, Ling L, ...
  • Kamata M, Amano H, Ito Y, Fujita T, Otaka F, ...
  • Han H, Zhu J, Wang Y, Zhu Z, Chen Y, ...
  • Ma W, Tao L, Wang X, Liu Q, Zhang W, ...
  • Wang D, Xiong M, Chen C, Du L, Liu Z, ...
  • Wen Y, Lu X, Ren J, Privratsky JR, Yang B, ...
  • Meng XM, Wang S, Huang XR, Yang C, Xiao J, ...
  • Wang YY, Jiang H, Pan J, Huang XR, Wang YC, ...
  • Yin Y, Liu W, Dai Y. SOCS۳ and its role ...
  • Gao Y, Zhao H, Wang P, Wang J, Zou L. ...
  • Pedroso JAB, Ramos-Lobo AM, Donato J. SOCS۳ as a future ...
  • White GE, Cotterill A, Addley MR, Soilleux EJ, Greaves DR. ...
  • Li S, Yu L, He A, Liu Q. Klotho inhibits ...
  • Yao L, Wright MF, Farmer BC, Peterson LS, Khan AM, ...
  • Colon S, Luan H, Liu Y, Meyer C, Gewin L, ...
  • Bhuvarahamurthy V, Kristiansen GO, Johannsen M, Loening SA, Schnorr D, ...
  • Reid AM, Juvonen R, Huuskonen P, Lehtonen M, Pasanen M, ...
  • Akbay P, Calis I, Undeger U, Basaran N, Basaran AA. ...
  • Attia YM, El-Kersh DM, Wagdy HA, Elmazar MM. Verbascoside: Identification, ...
  • Alipieva K, Korkina L, Orhan IE, Georgiev MI. Verbascosidea review ...
  • Paola RD, Oteri G, Mazzon E, Crisafulli C, Galuppo M, ...
  • Mechri B, Tekaya M, Hammami M, Chehab H. Root verbascoside ...
  • Yang L, Zhang B, Liu J, Dong Y, Li Y, ...
  • Khullar M, Sharma A, Wani A, Sharma N, Sharma N, ...
  • Hosseinian S, Shahraki S, Ebrahimzadeh Bideskan A, Shafei MN, Sadeghnia ...
  • Liu M, Zhu Y, Sun Y, Wen Z, Huang S, ...
  • Ma X, Chang Y, Xiong Y, Wang Z, Wang X, ...
  • Kinter M, Wolstenholme JT, Thornhill BA, Newton EA, McCormick ML, ...
  • Rouschop KM, Sewnath ME, Claessen N, Roelofs JJ, Hoedemaeker I, ...
  • Singh N, Shukla N, Singh P, Sharma R, Rajendran SM, ...
  • Xia D, Zhang Z, Zhao aY. Acteoside Attenuates oxidative stress ...
  • Wang L, Ren X, Tian XF, Cheng XL, Zhao YY, ...
  • Li Y, Liu J, Yu T, Yan B, Li H. ...
  • Wiezel D, Assadi MH, Landau D, Troib A, Kachko L, ...
  • Yang SX, Zhang ZC, Bai HL. ClC-۵ alleviates renal fibrosis ...
  • Tammaro A, Florquin S, Brok M, Claessen N, Butter LM, ...
  • Liu Y. Cellular and molecular mechanisms of renal fibrosis. Nat ...
  • Wang W, Zhou P-H, Hu W, Xu C-G, Zhou X-J, ...
  • de Moura Sperotto ND, Steffens L, Veríssimo RM, Henn JG, ...
  • Dimitrova P, Alipieva K, Stojanov K, Milanova V, Georgiev MI. ...
  • Spínola V, Castilho PC. Madeira moneywort (Sibthorpia peregrina L.) as ...
  • Jia WQ, Wang ZT, Zou MM, Lin JH, Li YH, ...
  • Li M, Zhou F, Xu T, Song H, Lu B. ...
  • Nishida M, Okumura Y, Fujimoto S-i, Shiraishi I, Itoi T, ...
  • Nan Si, Hajime Kanazawa, Katsuki Okuyama, Keisuke Imada, Hongjie Wang ...
  • Alston CI, Dix RD. SOCS and herpesviruses, with emphasis on ...
  • Illanueva EC, Myers MG, Jr. Leptin receptor signaling and the ...
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