Electrochemiluminescence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay), Tehran, Iran

Background and aims: Toxoplasmosis is a very common disease in the world and has two types,including chronic and acute toxoplasmosis. In the chronic toxoplasmosis, the abnormality of tissuefunction is negligible, but in acute toxoplasmosis, the function of the reticuloendothelial system isinterrupted and the assessment of Toxoplasma antibody with tissue enzymes is very essential in thisstage. In addition, in some conditions, serum ferritin increases in the acute phase of the infectiousdisease. In congenital toxoplasmosis, the evaluation of biochemical factors and IgG avidity test isimportant for detecting the acute congenital toxoplasmosis in pregnant women. Based on theabove-mentioned explanations, the present study aimed to evaluate the biochemical factors inpatients with acute toxoplasmosis (congenital toxoplasmosis) among the Iranian population usingelectrochemiluminescence and IgG ELISA avidity.Methods: The study was based on a comparative abundance study and was conducted from March toJune ۲۰۱۷. Material included ۹۸۰ serum and amniotic fluid samples collected from human blood witha high level of IgG antibody against Toxoplasma gondii in Rajaie Center, Tehran, Iran. The standard andthe main tests included the ELISA assay and the measurement of the liver transaminases (i.e., SGOTand SGPT), along with/namely bilirubin and ferritin used to detect IgG antibodies and to evaluate theacute toxoplasmosis, respectively. Finally, the results were analyzed by SPSS software.Results: The results showed that the level of liver transaminases, namely, serum bilirubin and ferritinincrease in some patients with a high level of IgG avidity antibody against Toxoplasma gondii. Forexample, the mean serum levels of SGOT was ۱۰۸ IU/L in ۱۲۰ patients with acute toxoplasmosis andthe mean serum bilirubin was about ۵ mg/dL in ۸۰ patients.Conclusion: Overall, in acute congenital toxoplasmosis, the evaluation of IgG AVIDITY is regarded asthe first step and then the measurement of biochemical factors such as serum transaminases, serumbilirubin, and serum ferritin is important.

کلیدواژه ها:

Toxoplasmosis pregnant women ، Electrochemiluminescence ، SGOT ، SGPT ، bilirubin ، ferritin ، IgG avidity