Assessment the bioactivity and biocompatibility of nanohydroxyapatite (n-HA) coated zein nanofiber for bone tissue regeneration

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 451

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

CIGS16_454

تاریخ نمایه سازی: 14 اردیبهشت 1400

چکیده مقاله:

Background and Aim: Bone tissue engineering is an exciting approach to directly repair bone defects or engineer bone tissue for transplantation. Biomaterials play a pivotal role in providing a template and extracellular environment to support regenerative cells and promote tissue regeneration. The attention given to zein based polymers is primarily attributed to their biocompatibility and biodegradability. It has been concluded that zein nanofibers have prolonged biodegradability period, which can be beneficial for fabrication of scaffolds in order to stimulate cell proliferation and differentiation in tissue engineering. However, due to the relatively low mechanical properties of these polymers, numerous inorganic compounds such as hydroxyapatite (HA) have been considered in combination with zein. HA has been widely used for bone regeneration because of its chemical similarity to natural bone. The aim of this study is assessment the bioactivity and biocompatibility of n-HA coated zein nanofiber for bone tissue regeneration.Methods: Zein scaffold was fabricated using electrospinning method. A polymer solution was prepared by dissolving zein in acetic acid. After fabrication, zein scaffolds coated with n-HA and analyzed with scanning electron microscopy (SEM). The chemical structure characterized by Fourier-transform infrared spectroscopy (FTIR) and Scaffold's mechanical properties evaluated by tensile test measurement. Mesenchymal stem cells (MSCs) cultured on these scaffolds in order to investigate cell viability and proliferation assay.Results: The analysis of SEM images was done with imagej software. The average dimeter of nanofibers was (۴۶۲±۲۶۰ nm) and (۵۴۷±۲۸۰ nm) in control and nHA coated zein mates respectively. The FTIR spectrum of samples had no significant difference in bulk zein and nanofibers. The results of MTT assay shows that the nHA coated zein nanofibers had better cell proliferation in respect with zein nanofibers.Conclusion: The highly porous Zein scaffolds showed improved mechanical properties, which might be applied as bone-cartilage substitution. Moreover n-HA has high biocompatibility, osteoconductivity, osteoinductivity, abrasion resistance, corrosion resistance, and stable chemical properties. The n-HA crystals on the nanofibers were randomly distributed and biomineralized nanofibers exhibited a positive influence on osteoblast growth. It was concluded that the zein-HA have potential for bone regeneration and tissue engineering.

کلیدواژه ها:

نویسندگان

Reza Sadeghi

Biology group, Faculty of Science, University of Guilan, Rasht, Iran

zivar salehi

Professor of biology group, Faculty of Science, University of Guilan, Rasht, Iran

mahvash hadavi

Biology group, Faculty of Science, University of Guilan, Rasht, Iran