The use of SSR and ISSR molecular markers to identify genetic diversity of different acid lime genotypes

Background and Aim: Limes are consumed as fresh fruits and processing products, due to high quality and acidity in fruit juice. In recent years, the prevalence of witches's broom disease has caused to loss thousands of lime trees in the south of Iran. The best method for controlling this disease is the cultivation of tolerant genotypes. The aim of this study was to investigate genetic diversity and to determine the relationship between some Mexican lime genotypes free from appearances of witches' broom disease collected in citrus collections in south of Iran using SSR and ISSR markers.Methods: In this study, genetic diversity of ۳۳ different lime genotypes with ۱۰ commercial citrus cultivars as a control was evaluated with ۱۷ pair primers of SSR and ۱۱ primers of ISSR.Results: According to Polymorphic Information Content (PIC) of SSR markers of TAA۱ and TC۲۶ with ۰.۷۲ and ۰.۶۸, respectively and using ISSR markers in ISSR۱, ۸۰۹ and ۸۱۰ all with ۰.۴۹, all of them were identified as a the most appropriate distinguishing markers. The results showed that there were differences between limes and other types of citrus based on kind of studied markers.Conclusion: All of limes genotypes had high affinity with Mexican lime and Persian lime and were grouped in a cluster. Therefore, the most of acid limes in south of Iran were belonged to limes group.

کلیدواژه ها:

Citrus ، Genetic diversity ، Lime ، Polymorphic Information Content