Effects of Different Extraction Solvents on Total Phenolics and Flavonoids Contents and Antioxidant Activities from C. procera Hairy Root Cultures

Background and Aim: Hairy root culture system as one of the valuable biotechnology tools for the production of secondary metabolites in medicinal herbs. It might be an attractive alternative over field-cultured plants as well as suspension cultures due to several advantages. C. procera is pharmaceutically important plant that exhibits significant antioxidant, anti-inflammatory, anti-bacterial, Insecticidal, antinociceptive and anticoagulant Properties. In the current study, the total phenolic and flavonoid contents as well as the amount of anodic acid of C. procera hairy roots in comparison with non-transgenic adventitious roots using different solvent system was investigated.Methods: In this study, hairy roots grown in phytohormone-free ۱/۲ MS medium as well as non-transgenic root from greenhouse-grown plants were harvested every week for ۵ weeks and subjected to the secondary metabolites analysis. Antioxidant capacity, total phenolic content and the amount of total flavonoid of the extracts were measured using free radical DPPH methods, reactive Folin-Ciocalteu and reagent aluminum chloride colorimetric method, respectively. It is worth noting that the measurements was carried out on methanol, ethanol, acetone and chloroform extracts of root materials.Results: The results showed that C. procera hairy roots had the highest amount of phenol, flavonoid contents and total antioxidants activities in all solvents in comparison with the control roots. The highest amount of total phenolic contents (۱۵.۸۸ mg gallic acid per gr of dry weight) and total flavonoid contents (۵.۹۵ mg quercetin per gr of dry weight) and antioxidant Properties (۲۴.۳۲%) were obtained in ۸۰% methanol extracts of hairy root. Among the solvents system, the chloroform had the lowest capability to extract the secondary metabolites.Conclusion: The results if current study showed that C. procera hairy roots are rich in phenolic and flavonoid compounds with higher antioxidant properties. According to the capable of rapid growth, genetic stability, and lack of geographical and time constraints, hairy roots are an important source in the production of secondary metabolites. The results indicated that the solvent type was found to significantly affect the recovery yields of secondary metabolites from the C. procera hairy root cultures.

کلیدواژه ها:

C. procera ، hairy root cultures ، secondary metabolites ، antioxidant properties ، solvent system.