microRNA-۱۴۳ inhibits metastasis of MDA-۲۳۱ breast cancer cell line

Background and Aim: Breast cancer (BC) is the most common cancer among females worldwide and the second cause of mortality associated with tumors after lung cancer. According to Global Cancer Statistics, approximately ۲.۱ million newly diagnosed cases of breast cancer in women will rise globally in ۲۰۱۸, accounting for about ۱ in ۴ cases of cancer in women. Given the advances in cancer therapy, this malignancy nevertheless appears incurable owing to metastasis and medication resistance. Nonetheless, studying the BC tumorigenesis process could be an effective way to overcome this issue. Over the past decades, the biology of microRNAs (a type of small non-coding RNA) has grown tremendously as potential therapeutic targets in cancer. miR-۱۴۳ is down-regulated in a wide range of human cancers including BC as a tumor suppressor miRNA and plays an important role in cell proliferation and metastasis. In various cancer cell lines, overexpression of miR-۱۴۳ inhibits cell proliferation, indicating that the absence of miR-۱۴۳ is observing in human cancers survival and amplifies tumor growth. Then miR-۱۴۳ replacement could be an effective therapeutic strategy.Methods: MDA-۲۳۱ cell lines purchased from Pasture Institute were cultivated in RPMI medium. In order to cell migration assay, MDA-۲۳۱ cells treated with miR-۱۴۳ mimics and they were seeded in ۲۴-well plate. After ۱۲ hours, we scratched the wells and the scratched area was quantified for ۲۴ hours. On the other hand in order to evaluate the expression levels of metastatic genes, we have assessed the expression level of Vimentin, MMP۳, and MMP۹ by qRT-PCR.Results: Overexpression of miR-۱۴۳ in BC cells compared with control cells inhibits metastasis through Vimentin, MMP۳, and MMP۹ down-regulation.Conclusion: According to our results, miR-۱۴۳ replacement through down-regulation of Vimentin, MMP۳, and MMP۹ may inhibit BC cell metastasis and be considered as a potential therapeutic strategy in BC treatment.

کلیدواژه ها:

Breast cancer ، miR-۱۴۳ ، metastasis