The effects of DNC on proliferation and telomere in mouse fibroblasts

Background and Aim: Curcumin (diferuloylmethane) is a hydrophobic bioactive ingredient found in a rhizome of the C. longa. Integrating curcumin into nanocarriers through various methods is an appropriate and fruitful choice to upsurge the biological activity of curcumin, which increases its bioavailability and solubility, long time circulation, and retention in the body, and overcome physiological barriers of curcumin. Also, it can reduce the unintended toxicity to surrounding normal cells/tissues by diffusing the indent tissues. Telomeres are essential for chromosomal integrity. Telomere shortening during cell division restricts cellular proliferative capacity and leads to cellular senescence. The aim of this study was to determine the effects of dendrosomal nanocurcumin (DNC) on cell proliferation induction in mouse embryonic fibroblast cell lines analyzed by MTT assay.Methods: In this study, We used several methods including fibroblast cell culture ,counting cell and seeding in a ۹۶ well plate and MTT assay.Treatment different concentrations of DNC for ۴۸ hours.perform mtt assay test and analysis with Graph pad software.Results: MEF cells under treatment of DNC with ۲ and ۴ µM showed increase in growth,proliferation and survival.Conclusion: In conclusion, nanocurcumin is effective on the proliferation and We observed a significant increase in the proliferation with DNC treatment of MEF cells, Therefore, our approach might be considered as a promising strategy for the development of new therapeutic modalities against aging.Further results will be presented on Congress Day.

کلیدواژه ها:

aging ، DNC ، Nanocurcumin ، telomere.proliferation