Molecular Diagnostics of Candida albicans -Secreted Aspartyl Proteinase Genes from women’s urinary tract infections yeast agent in Sari City

Background and Aim: Candidiasis is the most common fungal infectious disease. Candida albicans is the most important pathogenic Candida strain that can cause severe urinary tract infections in women. C. albicans secreted aspartyl proteinases (Sap) encoded by a family of ۱۰ genes. The function of Sap proteinases is important during infection and enhances the pathogenic ability of C. albicans. The aim of the present study were, molecular diagnosis of C. albicans genes on urinary tract infections agent in women in Sari City.Methods: Fifty of C. albicans isolates were obtained from patients with vaginitis symptoms who had referred to obstetrics and gynecology clinic in Sari City. DNA extraction was performed using a DNA extraction Kit and amplified by PCR to detect SAP genes with specific primers that were complementary to the C. albicans acting gene. The PCR product was sequenced and FASTA format of sequences were extracted using Chromas Lite software. After the determination of sequences, sequence analysis was performed by BLAST program in NCBI database. DNA sequences translated to amino acid sequences using sequence translation tools (EBI, (EXPASY Server). Then, the three-dimensional structure and the secondary and estimated protein structure were determined by PHYER۲ Protein Fold Recognition Server.Results: Results of homology comparison in the GenBank database using NCBI/BLAST shows C. albicans gene Sap۱ were ۹۷% homologous with C. albicans gene Sap۱ from Brazil (Bartelli et al., ۲۰۱۷). The C. albicans gene Sap۱ nucleotide sequence (۶۵۸ nucleotides) translated to ۲۲۰ amino acids sequences. Secondary structure of protein of C. albicans gene Sap۱ contains ۲۹% alpha helix and ۲۶% beta sheet structure. Results of homology comparison C. albicans gene Sap۳ shows ۹۷% homology similarity with C. albicans gene Sap۳ complete sequences from USA (White et al., ۲۰۰۸). The C. albicans gene Sap۳ nucleotide sequences (۶۶۴ nucleotides) translated to ۲۲۲ amino acids sequences.Conclusion: Conclusion of homology comparison C. albicans gene Sap۶ shows ۹۹% homology similarity with C. albicans Sap۶ gene for aspartyl protease from Switzerland ((Monod et al., ۱۹۹۴). C. albicans gene Sap۸ were ۹۴.۷۳% homologous with C. albicans gene Sap۸ from India (Rajpara, et al., ۲۰۱۸).

کلیدواژه ها:

Secreted Aspartyl Proteinase Gene ، Candida albicans ، Urinary Tract Infections ،