The meaningful change in Antibiotic-resistant gene expression of Staphylococcus aureus by using functionalized carbon nanotubes Nanofluid

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 239

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شناسه ملی سند علمی:

CIGS16_321

تاریخ نمایه سازی: 14 اردیبهشت 1400

چکیده مقاله:

Background and Aim: Staphylococcus aureus is a gram-positive bacterium and has been considered as an important nosocomial pathogen universal owing to its increasing antibiotic resistance and carries the Tet (M) gene encoding a tetracycline efflux pump. Recently, Carbon nanotubes have defined as novel and potential antibacterial and Nanocarrier agents. Aim: In this investigation, Antimicrobial effects study of carbon nanotubes Nanofluid on Staphylococcus aureus and gene expression study on the Tet (M) gene done.Methods: Cell viability assay was carried out after incubation of Staphylococcus aureus by the CNT's suspensions (۱۰۰ µg /ml) and antibiotic for ۲۴, ۴۸, and ۷۲ h. Then the gene expression profile of the Tet (M) gene was studied by Real-Time PCR method on bacteria, which were treated by functionalized carbon nanotubes Nano fluid+ Antibiotic in comparison with Antibiotic alone.Results: Results were conferred significantly, reduced bacterial proliferation, and reduced expression of the Tet (M) gene in the Nano fluid+ Antibiotic condition.Conclusion: The present study showed that the functionalized carbon nanotubes Nanofluid could be a proper drug delivery vehicle for overcoming bacterial Antibiotic-resistant.

نویسندگان

Hengameh Jannati

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.

Mojgan Sheikhpour

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran

Mohammad Reza Yazdani

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran