The value of Fibroblast Growth Factor ۱۰ and novel FGF۱۰AS as a prognostic biomarker in colorectal cancer patients

Background and Aim: Colorectal cancer (CRC) imposes great health burdens around the world. Growth factors contribute to cell growth, differentiation, angiogenesis, and most importantly tumor formation in many types of cancers. Natural antisense transcripts (NATs) are inclusively expressed in the genome and are predicted to play a major role in cancer progression. The present study aimed at evaluating the relationship of fibroblast growth factor ۱۰ (FGF۱۰) and FGF۱۰AS expression with clinicopathological features in CRC progression.Methods: This study was navigated on ۱۵۰ CRC tumor tissues and ۱۵۰ adjacent non-tumor tissues according to the inclusion and exclusion criteria. The expression levels of FGF۱۰ and FGF۱۰AS were evaluated by the strand-specific real-time quantitative polymerase chain reaction (RT-qPCR). Immunohistochemistry analysis was performed systematically for evaluating FGF۱۰ expression. The collected data were analyzed using Prism ۸.۰۲ and SPSS ۲۳.Results: A significant increase and decrease in expression levels were observed in FGF۱۰ (P< ۰.۰۰۰۱) and FGF۱۰AS (P> ۰.۰۱) in tumoral tissues, in comparison with the adjacent normal tissues. Besides, overexpression of FGF۱۰ and diminution in FGF۱۰AS expression were strongly correlated with the TNM stage (P< ۰.۰۰۵۱ and P< ۰.۰۲, respectively) and vascular invasion (P< ۰.۰۳ and P< ۰.۰۱, respectively). Moreover, the area under the ROC curve for the prognostic value of FGF۱۰ was about ۰.۸۹ (۹۵% confidence interval, ۰.۸۷۷–۰.۹۴۱). The results of linear regression analysis confirmed a negative correlation between FGF۱۰ expression and its antisense transcript (r= -۰.۰۲).Conclusion: The correlation between the expression levels of FGF۱۰ and FGF۱۰AS in tumor tissues and the adjacent normal tissues suggested that sense and antisense FGF RNAs could be significant prognostic biomarkers for elevating survival rates and treatment improvement.

کلیدواژه ها:

Fibroblast growth factor ۱۰ ، Long non-coding RNA ، Colorectal neoplasms ، biomarker