Comparison of the expression profile of miR-۴۹۰-۵P transcripts in healthy samples with Glioblastoma multiforme tumor using MicroRNA sequencing

Background and Aim: Glioblastoma multiforme (GBM) is one of the most common and aggressive types of malignant central nervous system tumors. Common multivariate therapies such as surgery, radiation therapy and chemotherapy cannot control this tumor well. The most invasive one is Grade ۴ or GBM. Since some cancers have genetic conditions and genes are involved, the Next Generation Sequencing (NGS) technique is used for gene sequencing and diagnosis. Using this technique, molecular properties of the tumors are obtained. The miRNAs are a group of non-coding ۱۸-۲۵ nucleotide RNAs that have been kept in terms of evolution. The main symptoms of cancer such as cellular differentiation, cellular proliferation, cellular cycle, cellular survival and cellular invasion are regulated by miRNAs Methods: In this study, RNA extraction from both GBM tissue and normal brain tissue was done. After qualitative evaluation, cDNA synthesis was performed. Next, samples were sent to China for next generation sequencing. After the analysis of responses, one of miRNA; miR-۴۹۰-۵P, which has various roles in other cancers, was selected from the list of miRNAs. The primer was designed and real-time PCR technique was used for confirming.Results: After sequencing, the results were evaluated using bioinformatics software. Considering the increase and decrease of microRNAs expression in the healthy and patient samples, the desired miRNA was selected. We confirm that the expression of miR-۴۹۰-۵P is increased in GBM tissue, in comparison with normal brain tissue and significant relationship was found between gender and age Conclusion: Increasing the expression of miR-۴۹۰-۵P can play a role in GBM by affecting gene targets and routes and a role in the diagnosis of glioblastoma as a marker and recognizing early stages of the disease

کلیدواژه ها:

Glioblastoma Multiforme ، MicroRNA sequencing. Next Generation Sequencing ، Real-time PCR