Evaluation of the expression of NPTN-IT۱ Long non-coding RNA in breast cancer and its relationship with clinicopathological information and reproductive factors

Background and Aim: Breast cancer causes the highest related death among women. Long non coding RNAs (lncRNAs), a subclass of non-coding RNAs, which are involved in significant functions in regulation of gene expression. However the tumor suppressive roles of lncRNA NPTN-IT۱ has been identified in some cancers, its expression in breast tumors and normal tissues is still unknown. In this study, we aimed to assess the expression of lncRNA NPTN-IT۱ in breast tumors and normal tissues, and in these contexts we investigate the potential lncRNA NPTN-IT۱ /miRNA/mRNA axes.Methods: The expression of lncRNA NPTN-IT۱ was analyzed using real-time RCR. Also, we assessed the association of lncRNA NPTN-IT۱ expression with demographic and clinicopathological information of patients. Then, a bioinformatics approach was used to revealed the critical lncRNA NPTN-IT۱ -mediated sponge regulatory network.Results: lncRNA NPTN-IT۱ expression was downregulated in both breast cancer tissues and adjacent normal tissues. Also, it was found that decreased expression of lncRNA NPTN-IT۱ is significantly related to early-onset menarche and late-onset menopause. The bioinformatics analyses proving that lncRNA NPTN-IT۱ has function as a tumor suppressor sponge.Conclusion: Together, these results indicate that lncRNA NPTN-IT۱ shows a significant down expression in breast cancer tissues and adjacent normal tissues compared with normal mammary tissues. These data suggest that normal breast tissues can undergo tumor-related molecular changes. In silico analyses show that lncRNA NPTN-IT۱ plays an essential role in transcriptional misregulation in various malignancies.

کلیدواژه ها:

lncRNA NPTN-IT۱ ، Breast cancer ، Expression ، ceRNA