The study of miR-۳۲۰a expression in multiple sclerosis patients in Zanjan

Background and Aim: Multiple sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system origin (CNS). In recent years, the rate of developing the disease has increased in the country. New medical advancements indicate the presence of genetic and environmental factors on the disease. MicroRNAs as important regulators of gene expression at the post-transcriptional stage has revealed another level of regulate gene expression complexity. Their performance is essential for homeostasis safety and prevention of autoimmune diseases. Recent MS studies are revealing that the microRNAs expression profiles are disturbed, so the microRNAs can be used as a biomarker for risk assessment, monitor the treatment process and finally, early detection. There is a possibility that the unset expression of micro-RNAs lead to break the immune tolerance and autoimmune diseases. One of the microRNAs that its role in inflammatory and autoimmune diseases and cancer has been confirmed, is miR-۳۲۰a which in different diseases has different sets and expressions. miR-۳۲۰a targets mRNA matrix metallopeptidase-۹ in MS patients, decreases expression of this micro-RNA, increases the expression of matrix metallopeptidase-۹ and ultimately increases permeability of the blood-brain barrier and neurological disability. The aim of this study was to investigate changes in the expression of miR-۳۲۰a in the plasma of MS patients.Methods: RNA was extracted from isolated blood plasma of ۲۴ MS patients and ۳۰ healthy individuals. cDNA was synthesized by primer Specialized Stem-Loop, miR-۳۲۰a and expression levels was investigated using miR-۱۶ as a reference gene and qReal time-PCR technique.Results: The extracted data were evaluated by the Ct?? comparative method. The results of changes in the miR-۳۲۰a expression, standard deviation and p-vaiue in different groups were obtained. All the experiments were repeated ۳ times and duplicated. The final data were transferred to the OpenEpi software and the means were compared using the T-test method with P <۰.۰۵. (۰.۱۷۷۱۸۸۲۳۶ ± ۰.۳۲۵۷۳۹۷۷) and (P-value <۰.۰۵). Of the ۲۴ MS patients studied, in ۲۳ cases we observed a decrease in miR-۳۲۰a expression compared to the control group.Conclusion: A significant decrease in expression of miR-۳۲۰a in the plasma of patients with MS was observed compared to the control group. The results of this study showed that the expression levels of miR-۳۲۰a in the plasma has fallen. This reduction is important in the pathogenesis of MS.

کلیدواژه ها:

Multiple sclerosis ، qReal time-PCR ، Stem-Loop ، miR-۳۲۰a