Evaluation Of VEGF And FGF Gene Expression Of Chicken'sEmbryo Angiogenesis Affected By Doxycycline Antibiotic

Background and Aim: VEGF and FGF genes expression have designated as vascular endothelial and fibroblast growth factors, respectively which both have great role on angiogenesis. Angiogenesis, the growth of new vessels from exiting is one of the most important biological events such as physiological events in embryonic development or pathological events including the growth of tumor was observed. Doxycycline has a wide biomedical applications as compared to other antibiotics. The aim of this study was to investigate the effect of doxycycline antibiotic on VEGF and FGF genes expression of chorioallantoic membrane angiogenesis of chicken's embryo.Methods: ۴۰ fertilized eggs(Ross) were randomly divided to ۵ groups and encubated. On the second day of incubation, the window was created for all the groups on the surfaces of egg shell. On the eighth day of incubation, by putting gelatin sponge on chorioalantoic membrane , in control group, ۱۰μl distilled water, in experimental group ۱: ۱۰ μl doxycycline (۵۰ μmol/ml) and experimental group ۲ : ۱۰ μl doxycycline (۲۵۰ μmol/ml) were treated. In ۱۲th day, chorioalantoic membrane of all samples captured and the number and length vessels were measured. The weight and height of the fetus were investigated and the vessels were removed to examine the expression of VEGF and FGF gene expression. Extracted RNA, cDNA synthesis and Real-Time were performed.Results: Results of statistical analysis showed that the number and length of vessels in the experimental groups compared with control groups were significantly decreased. The length and weight of the fetuses had no significant differences, however these parameters in the experimental group ۳, had significantly decreased. Expression of VEGF and FGF in the experimental groups compared to control were decreased.Conclusion: According to the results of this study, doxycycline antibiotic probably with inhibitory effects on gene expression of VEGF and FGF, decreased chorioallantoic membrane angiogenesis of chicken's embryo but had no effect on growth and embryonic development in this concentration.

کلیدواژه ها:

Angiogenesis ، Doxycycline Antibiotic ، Chorioallantoic membrane ، FGF ، VEGF