Chronic Caffeine Ingestion Down-Regulates Liver and Visceral Adipose Tissue Inflammatory Gene Expression in High-Fat Diet-Induced Obesity

Background and Objective: Beverages containing caffeine have an anti-obesity function. Reduction of visceral adipose tissue (VAT) inflammation is considered an important strategy to ameliorate obesity compilations such as insulin resistance. This study aimed to investigate the effect of ۸-week caffeine supplementation on the messenger RNA (mRNA) expression of fetuin-A (FetA) in the liver and nuclear factor kappa B (Nf-κb) and toll-like receptor ۴ (Tlr۴) in the VAT of rats with a high-fat diet (HFD). Methods: A total of ۴۰ male Wistar rats were randomly divided into control, caffeine, HFD, and HFD+caffeine supplement groups. After ۲ weeks of acclimatization, the rats were randomly fed with HFD (۴۶% fat) and a normal diet (۵% fat) for ۸ weeks. The rats in the caffeine groups were gavaged with ۶ mg of the caffeine solution per kg of body weight. FetA mRNA of the liver, Nf-κb, and Tlr۴ mRNA of VAT were determined using real-time polymerase chain reaction (PCR). Results: The results indicated that FetA mRNA expression and weight gain in HFD+caffeine were significantly reduced compared to the other groups. Nf-κb mRNA expression was significantly higher in the HFD group than in the caffeine groups. No statistically significant differences were found in Tlr۴ mRNA expression between the groups. Conclusion: These findings suggest that consuming caffeine can prevent HFD-induced liver and adipose tissue (AT) inflammation.

کلیدواژه ها:

High-fat diet ، Caffeine ، Adipose tissue ، Inflammation ، Fetuin-A ، Toll-like receptor ۴ ، Nuclear factor kappa B