The Comparison of Green Tea Aqueous Extract and Catechin Effect on Pituitary-Gonadal Axis in Rat Models of Type ۱ Diabetes

Background and Objective: Diabetes causes fertility disorders by interfering with the endocrine gland function. There are reports that, green tea and catechins could have anti-oxidant and hypoglycemic properties. Therefore, in the present study, we evaluated the effects of green tea aqueous extract and catechin influence on pituitary-gonadal axis in rat models of type ۱ diabetes. Materials & Methods: Six groups of Wistar rats (۸ in each group), including control, diabetic control (intraperitoneal injection (IP) of ۰.۵ mL saline solution for ۳۰ days after induction of diabetes), diabetic treated with ۱۰۰ and ۲۰۰ mg/kg doses of green tea aqueous extract (IP injection of ۰.۵ mL green tea extract for ۳۰ days), and diabetic treated with ۱۰۰ and ۲۰۰ mg/kg doses of catechin (IP injection ۰.۵ mL of catechin for ۳۰ days) were used. The induction of diabetes was conducted through an IP injection of ۲۴۰ mg/kg alloxan. At the end of the treatment course, the serum levels of LH, FSH, estrogen, testosterone, dihydrotestosterone and cytoplasmic HOdG-۸ in testicular tissue were measured by ELISA method. ANOVA and Tukey post hoc test (P<۰.۰۵) were used to perform the data analysis. Results: The incorporation of ۲۰۰ mg/kg green tea extract and ۱۰۰ and ۲۰۰ mg/kg concentrations of catechin, in comparison with the diabetic control group, led to a significant dose-dependent increase in the serum level of LH, FSH, estrogen, testosterone, and dihydrotestosterone. A dose-dependent significant decrease was observed in HOdG-۸ in the testicular tissue of diabetic rats (P<۰.۰۵).   Conclusion: Based on the obtained data, compared to green tea, catechin considerably enhanced the hormonal parameters and reduced HOdG-۸ in testicular tissue of diabetic rats.

کلیدواژه ها:

Catechin ، Diabetes mellitus ، Green tea ، Oxidative Stress ، Rats ، Testis