The Antiapoptotic Effect of Ghrelin in the H۲O۲ Treated Bone Marrow-Derived Mesenchymal Stem Cells of Rat

Background and Objective: Apoptosis is a form of programmed cell death and oxidative stress is one of the factors that lead to apoptosis. Ghrelin is a ۲۸ amino acid peptide and its antiapoptotic effects have been shown previously. The aim of this study was to evaluate the effect of ghrelin on cell survival and apoptosis of rat bone marrow mesenchymal stem cells (BMSCs). Materials and Methods: BMSCs were cultured as control and H۲O۲ treated groups with and without different doses of ghrelin (۰.۱-۱-۱۰-۱۰۰ µM) for ۲۴ and ۴۸ hours. To find out the best dose and time of effect for ghrelin, MTT was used to determine cell survival. BMSC apoptosis rate was detected using the TUNEL technique. Results: Cultured BMSCs showed that the cells are fibroblastic in appearance and adherent to culture dishes as well as being capable of having a very high proliferation rate. Ghrelin treatment increased proliferation and cell survival in both normal and H۲O۲ exposed BMSCs. The best dose and time that ghrelin could affect cell proliferation was ۱۰۰µM and ۴۸ hours respectively. Treatment with ghrelin significantly reduced apoptosis in BMSCs in this dose and time. Conclusion: Ghrelin treatment causes an increase in BMSCs proliferation and reduction of H۲O۲ induced apoptosis in these cells.    

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Keywords: Apoptosis ، Ghrelin ، H۲O۲ ، BMSC ، Rat