The Antitumoral Activity of Zataria Multiflora Methanolic Extract on Acute Promyelocytic Leukemia Cell Line; NB۴

Background & Objective:  Zataria multiflora is a plant that belongs to Laminaceae family. It is traditionally believed to have several therapeutic effects. Acute promyelocytic leukemia is a distinct subtype of acute myeloid leukemia with dominancy of promyelocytes in bone marrow and blood stream. The aim of this study is to investigate the anticancer effects of Z. multiflora extract on acute promyelocytic leukemia cell lines. Materials & Methods:  Viability of NB۴ cells was determined by trypane blue test after treatment with ۲۰, ۴۰ and ۸۰µg/mL of Z. multiflora extract for ۲۴ hours. Then, the metabolic activity of cells was determined by MTT assay after ۲۴ hours of treatment with ۸۰ µg/mL Z. multiflora. Finally, Real-time PCR was employed to study the effect of Z. multiflora extract on the expression of hTERT gene. Results:  Z. multiflora extract decreased the viability of NB۴ cells in a dose-dependent manner. Metabolic activity of NB۴ cells significantly decreased following treatment with ۸۰ µg/mL Z. multiflora. Gene expression analysis showed ۵۹%±۴% decrease in the expression of hTERT gene after treatment with ۸۰ µg/mL of Z. multiflora. Conclusion:  Z. multiflora extract significantly decreased the viability and metabolic activity of NB۴ cells. It also led to significant downregulation of hTERT gene compared to the control group. Therefore, Z. multiflora methanolic extract potentially has anticancer effect on acute promyelocytic leukemia cells through down regulation of hTERT. Further investigations are needed to explore other mechanisms of actions and the active ingredients.

کلیدواژه ها:

Zataria multiflora ، Tumor Suppressor ، Leukemia ، Acute Promyelocytic Leukaemia ، NB۴ ، hTERT gene