The Neuroprotective Effect of Pretreatment with Lavandula officinalis Ethanolic Extract on Brain Edema in Rat Stroke Model

Background and Objective: Clinically significant changes in central nervous system (CNS) function are observed following brief periods of CNS blood flow interruption. Stroke patients exhibit cognitive, emotional, and electrophysiological changes during the recovery phase. Lavender belongs to the Labiatae family and is used for a variety of cosmetic and therapeutic purposes in herbal medicine. This study investigates the effect of ethanolic extract of lavender on cerebral edema in a rat stroke model. Materials and Methods: Lavender extract was injected intraperitoneally (۱۰۰ mg/kg and ۲۰۰ mg/kg) for ۲۰ consecutive days. ۲ hours after the last dose cerebral artery ligation surgery was performed. ۲۴ hours after the induction of ischemia, cerebral edema was assessed. Also, antioxidant capacities of plasma and brain tissue were assessed in the intact groups. Results: This study shows that treatment of rats with ethanolic extract of lavender caused a significant decrease in brain water content compared with ischemia group. Lavender extracts increased antioxidant capacity in serum and brain tissue in the intacts compared with the control group. Conclusion: The results indicated that lavender extract has neuroprotective activity against cerebral ischemia and the mechanism may be related to augmentation in endogenous antioxidant defense and inhibiting oxidative stress in the rat brain. References ۱- Bhuiyan MIH, Kim YJ. Mechanisms and prospects of ischemic tolerance induced by cerebral preconditioning. International Neurourology Journal. ۲۰۱۰ ۱۴: ۲۰۳. ۲- Lakhan SE, Kirchgessner A, Hofer M. Inflammatory mechanisms in ischemic stroke: therapeutic approaches. J Transl Med. ۲۰۰۹ ۷: ۹۷. ۳- Warner DS, Sheng H, Batinić-Haberle I. Oxidants, antioxidants and the ischemic brain. J Exp Biol. ۲۰۰۴ ۲۰۷: ۳۲۲۱. ۴- Crack PJ, Taylor JM. Reactive oxygen species and the modulation of stroke. Free Radic Biol Med. ۲۰۰۵ ۳۸: ۱۴۳۳-۴۴. ۵- Chan PH. Reactive oxygen radicals in signaling and damage in the ischemic brain. J Cereb Blood Flow Metab. ۲۰۰۱ ۲۱: ۲-۱۴. ۶- Abramov AY, Scorziello A, Duchen MR. Three distinct mechanisms generate oxygen free radicals in neurons and contribute to cell death during anoxia and reoxygenation. J Neurosci ۲۰۰۷ ۲۷: ۱۱۲۹-۳۸. ۷- Moopanar TR, Allen DG. Reactive oxygen species reduce myofibrillar Ca۲+ sensitivity in fatiguing mouse skeletal muscle at ۳۷ C. J Physiol. ۲۰۰۵ ۵۶۴: ۱۸۹-۹۹. ۸- Vincent AM, Russell JW, Low P, Feldman EL. Oxidative stress in the pathogenesis of diabetic neuropathy. Endocr Rev. ۲۰۰۴ ۲۵: ۶۱۲-۲۸. ۹- Simard JM, Kent TA, Chen M, Tarasov KV, Gerzanich V. Brain oedema in focal ischaemia: molecular pathophysiology and theoretical implications. Lancet. ۲۰۰۷ ۶: ۲۵۸-۶۸. ۱۰- Omidbaigi R. Production and processing of medicinal plants. Astane Ghods Publications, Mashhad. ۲۰۰۰ ۳: ۱۰۶-۲۲. ۱۱- Hajhashemi V, Ghannadi A, Sharif B. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia mill. J Ethnopharmacol. ۲۰۰۳ ۸۹: ۶۷-۷۱. ۱۲- Rabiei Z, Rafieian-Kopaei M, Mokhtari S, Alibabaei Z, Shahrani M. The effect of pretreatment with different doses of Lavandula officinalis ethanolic extract on memory, learning and nociception. Biomedicine & Aging Pathology. ۲۰۱۳ ۴: ۷۱-۷۶. ۱۳- Bigdeli MR, Hajizadeh S, Froozandeh M, et al. Normobaric hyperoxia induces ischemic tolerance and upregulation of glutamate transporters in the rat brain and serum TNF-α level. Exp Neurol. ۲۰۰۸ ۲۱۲: ۲۹۸-۳۰۶. ۱۴- Rabiei Z, Rafieian M. Effects of Zizyphus jujuba extract on motor coordination impairment induced by bilateral electric lesions of the nucleus basalis of meynert in rat. Physiology and Pharmacology. ۲۰۱۳ ۱۷: ۴۶۹-۷۷. ۱۵- Benzie IF, Strain J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. ۱۹۹۶ ۲۳۹: ۷۰-۶. ۱۶- Abdollahi M, Mostafalou S, Pournourmohammadi S, Shadnia S. Oxidative stress and cholinesterase inhibition in saliva and plasma of rats following subchronic exposure to malathion. Comparative biochemistry and physiology part C: Toxicol Pharmacol. ۲۰۰۴ ۱۳۷: ۲۹-۳۴. ۱۷- Astrup J. Energy-requiring cell functions in the ischemic brain: their critical supply and possible inhibition in protective therapy. J Neurosurg. ۱۹۸۲ ۵۶: ۴۸۲-۹۷. ۱۸- Quast MJ, Huang NC, Hillman GR, Kent TA. The evolution of acute stroke recorded by multimodal magnetic resonance imaging. Magnetic Resonance Imaging. ۱۹۹۳ ۱۱: ۴۶۵-۷۱. ۱۹- Wang Y, Hu W, Perez-Trepichio AD, et al. Brain tissue sodium is a ticking clock telling time after arterial occlusion in rat focal cerebral ischemia. Stroke. ۲۰۰۰ ۳۱: ۱۳۸۶-۹۲. ۲۰- Rami A, Langhagen A, Steiger S. Focal cerebral ischemia induces upregulation of beclin ۱ and autophagy-like cell death. Neurobiol Aging. ۲۰۰۸ ۲۹: ۱۳۲-۴۱. ۲۱- Taghikhani M, Nasri H, Asgari A, et al. The renal toxicity of hydroalcoholic extract of stachys lavandulifolia vahl in wistar rats. Life Sci J. ۲۰۱۲ ۹: ۳۰۲۵-۳۱. ۲۲- Coyle JT, Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science. ۱۹۹۳ ۲۶۲: ۶۸۹-۹۵. ۲۳- Alnamer R, Alaoui K, Bouididael H, Benjouad A, Cherrah Y. Sedative and hypnotic activities of the methanolic and aqueous extracts of Lavandula officinalis from Morocco. Adv Pharmacol Sci. ۲۰۱۲ ۵: ۱-۵. ۲۴- Rahmati B, Khalili M, Roghani M, Ahghari P. Anti-epileptogenic and antioxidant effect of Lavandula officinalis aerial part extract against pentylenetetrazol-induced kindling in male mice. J Ethnopharmacol. ۲۰۱۳ ۱۴۸: ۱۵۲-۷. ۲۵- Mohagheghi F, Bigdeli MR, Rasoulian B, Zeinanloo AA, Khoshbaten A. Dietary virgin olive oil reduces blood brain barrier permeability, brain edema, and brain injury in rats subjected to ischemia-reperfusion. Sci W J. ۲۰۱۰ ۱۰: ۱۱۸۰-۹۱. ۲۶- Baradaran A, Madihi Y, Merrikhi A, Rafieian-Kopaei M, Nasri H. Serum lipoprotein (a) in diabetic patients with various renal function not yet on dialysis. Pak J Med Sci. ۲۰۱۳ ۲۹: ۳۳-۵. ۲۷- Nasri H. Impact of diabetes mellitus on parathyroid hormone in hemodialysis patients. J parathyr dis. ۲۰۱۴ ۱: ۹-۱۱. ۲۸- Madihi Y, Merrikhi A, Baradaran A, et al. Impact of sumac on postprandialhigh-fat oxidative stress. ۲۰۱۳. in press. ۲۹- Setorki M, Rafieian-Kopaei M, Merikhi A, et al. Suppressive impact of anethum graveolens consumption on biochemical risk factors of atherosclerosis in hypercholesterolemic rabbits. J parathyr dis. ۲۰۱۳ ۴: ۸۸۹. ۳۰- Khosravi-Boroujeni H, Sarrafzadegan N, Mohammadifard N, et al. White rice consumption and CVD risk factors among Iranian population. J Health Popul Nutr. ۲۰۱۳ ۳۱: ۲۵۲. ۳۱- Akhlaghi M, Shabanian G, Rafieian-Kopaei M, Parvin N, Saadat M, Akhlaghi M. Citrus aurantium blossom and preoperative anxiety. Rev Bras Anestesiol. ۲۰۱۱ ۶۱: ۷۰۷-۱۲. ۳۲- Roohafza H, Sarrafzadegan N, Sadeghi M, Rafieian-Kopaei M, Sajjadi F, Khosravi-Boroujeni H. The association between stress levels and food consumption among iranian population. Arch Iran Med . ۲۰۱۳ ۱۶: ۱۴۵-۸. ۳۳- Azadmehr A, Hajiaghaee R, Afshari A, et al. Evaluation of in vivo immune response activity and in vitro anti-cancer effect by Scrophularia megalantha. J Med Plants Res. ۲۰۱۱ ۵: ۲۳۶۵-۸. ۳۴- Shirzad H, Shahrani M, Rafieian-Kopaei M. Comparison of morphine and tramadol effects on phagocytic activity of mice peritoneal phagocytes in vivo. Int Immunopharmacol. ۲۰۰۹ ۹: ۹۶۸-۷۰. ۳۵- Amini FG, Rafieian-Kopaei M, Nematbakhsh M, Baradaran A, Nasri H. Ameliorative effects of metformin on renal histologic and biochemical alterations of gentamicin-induced renal toxicity in wistar rats. J Res Med Sci. ۲۰۱۲ ۱۷: ۶۲۱. ۳۶- Nasri H, Nematbakhsh M, Ghobadi S, Ansari R, Shahinfard N, Rafieian-kopaei M. Preventive and curative effects of ginger extract against histopathologic changes of gentamicin-induced tubular toxicity in rats. Prev Med. ۲۰۱۳ ۴: ۳۱۶. ۳۷- Heidarian E, Rafieian-Kopaei M. Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat. Pharm Biol. ۲۰۱۳ ۵۱: ۱۱۰۴-۹. ۳۸- Shirzad H, Taji F, Rafieian-Kopaei M. Correlation between antioxidant activity of garlic extracts and WEHI-۱۶۴ fibrosarcoma tumor growth in BALB/c mice. J Med Food. ۲۰۱۱ ۱۴: ۹۶۹-۷۴. ۳۹- Nikokar M, Shirzad M. Does royal jelly affect tumor cells? J Herb Med Pharmacol. ۲۰۱۴ ۲: ۴۵-۴۸. ۴۰- Rafieian-Kopaei M, Baradaran A, Rafieian M. Plants antioxidants: From laboratory to clinic. J Nephropathol. ۲۰۱۳ ۲: ۱۵۲.

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Keywords: Brain edema ، Brain stroke ، Rat