Immunomodulatory Effects by Hydroalcoholic Liquorice Root Extracts

سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 228

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شناسه ملی سند علمی:

JR_ZUMS-22-95_011

تاریخ نمایه سازی: 11 اردیبهشت 1400

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Background and Objective: The roots of Glycyrrhiza glabra (Liquorice) have long been used in traditional medicine to treat a variety of ailments from simple coughs to hepatitis and even more complexities such as cancers. This study was conducted to grasp the immunomodulatory properties of the roots of Glycyrrhiza glabra in NMRI-mice challenged with sheep red blood cells (SRBCs). Materials and Methods: The study population consisted of ۲۰ male mice that randomly divided into two equal groups and immunized with SRBC. Mice in the treatment group orally received ۰.۷۵ g/Kg hydroalcoholic extract of Glycyrrhiza glabra every day from the beginning of the study for ۲ weeks. Results: The results indicated a significant increase in the level of anti-SRBC antibody and simultaneously a significant decrease in the level of cellular immunity in the treatment group compared to the control group. Moreover, the level of the respiratory burst of phagocyte cells of splenocytes significantly decreased in the treatment groups, while the level of lymphocyte proliferation significantly increased in the treatment group as compared to the control group. Conclusion: Hydroalcoholic root extract of Glycyrrhiza glabra may be used as a natural source in immune system. References ۱- Amani M, Sotudeh-Gharebagh R, Mostaoufi N, Kashani H. Optimal exteraction of glycyrrhetinic acid from licorice root. Technol. ۲۰۰۵ ۳(۴): ۳۷۶-۵۸۰. ۲- Nezamabadi H, Rahimiyan Mashhadi H, Zand A and Alizadeh H. Ecophysiological aspects of licorice rhizome. Plant Dis Pests. ۱۳۸۵ ۷۴(۲): ۴۵-۶۲. ۳- Marzi V, Circella G, Vampa GM. Effect of soil depth on the rooting system growth in Glycyrrhiza glabra L. Acta Horti. ۱۹۹۳ ۳۳۱: ۷۱-۸. ۴- Nassirasl M and Hosseinzadeh H. Review of pharmacological effects ofglycyrrhiza sp. and its bioactive compounds. Phytother Res. ۲۰۰۸ ۲۲: ۷۰۹-۷۲۴. ۵- Armanini D, Mattarello MJ, Fiore C, et al. Licorice reduces serum testosterone in healthy women. Steroids. ۲۰۰۴ ۶۹(۱۱-۱۲): ۷۶۳-۶. ۶- de Paula FT, Frauches PQ, Pedebos C, et al. Improving the thrombin inhibitory activity of glycyrrhizin, a triterpenic saponin, through a molecular simplification of the carbohydrate moiety. Chem Biol Drug Des. ۲۰۱۳ ۸۲(۶): ۷۵۶-۶۰. ۷- Mendes-Silva W, Assafim M, Rata B, Monteiro RQ, Guimaraes JA, Zingalia RB. Antithrombiotic effect of glycyrrihizin, a plant derived thrombin inhibitor. Thromb Res. ۲۰۰۳ ۱۱۲: ۹۳-۹۸. ۸- Su EY, Fang YH, Chen HS. Clinical observation of treating ۶۲ patients with severe aplastic anemia failing in immunosuppressive therapy by integrative medicine. Zhongguo Zhong Xi Yi Jie He Za Zhi. ۲۰۱۲ ۳۲(۱۲): ۱۶۱۶-۲۰. ۹- Methlie P, Husebye EE, Hustad S, Lien EA, Lovas K. Grapefruit juice and licorice increase cortisol availability in patients with Addisonchr('۳۹')s disease. Eur J Endocrinol. ۲۰۱۱ ۱۶۵(۵): ۷۶۱-۹. ۱۰- Cooper H, Bhattacharya B, Verma V, McCulloch AJ, Smellie WS, Heald AH. Liquorice and soy sauce, a life-saving concoction in a patient with Addisonchr('۳۹')s disease. Ann Clin Biochem. ۲۰۰۷ ۴۴(Pt ۴): ۳۹۷-۹. ۱۱- Fuhrman B, Volkova N, Kaplan M, et al. Antiatherosclerotic effects of licorice extract supplementation on hypercholesterolemic patients: increased resistance of LDL to atherogenicmodifications, reduced plasma lipid levels, and decreased systolic blood pressure. Nutrition. ۲۰۰۲ ۱۸(۳): ۲۶۸-۷۳. ۱۲- He X, Li X, Liu B, Xu L, Zhao H, Lu A. Down-regulation of Treg cells and up-regulation of TH۱/TH۲ cytokine ratio were induced by polysaccharide from radix glycyrrhizae in H۲۲ hepatocarcinoma bearing mice. Molecules (Basel, Switzerland). ۲۰۱۱ ۱۶(۱۰): ۸۳۴۳-۵۲. ۱۳- Kim J, Joo I, Kim H, Han Y. ۱۸beta-glycyrrhetinic acid induces immunological adjuvant activity of Th۱ against Candida albicans surface mannan extract. Phytomedicine. ۲۰۱۳ ۲۰(۱۱): ۹۵۱-۵. ۱۴- Zimecki M, Wieczorek Z. Differential patterns of cyclosporine A-induced inhibition of humoral and cellular immune responses to sheep erythrocytes in mice. Polish J Pharmacol. ۲۰۰۱ ۵۳(۵): ۴۹۵-۵۰۰. ۱۵- Hassan ZM, Ebtekar M. Immunological consequence of sulfur mustard exposure. Immunol Lett. ۲۰۰۲ ۸۳(۳): ۱۵۱-۲. ۱۶- Abtahi Froushani SM, Delirezh N, Hobbenaghi R, Mosayebi G. Synergistic effects of atorvastatin and all-trans retinoic acid in ameliorating animal model of multiple sclerosis. Immunol Invest. ۲۰۱۴ ۴۳(۱): ۵۴-۶۸. ۱۷- Esmaili Gouvarchin galeh H, Delirezh N, Abtahi Froushani SM, Afzale Ahangaran N. Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions. Turk J Biol. ۲۰۱۴ ۳۸: ۳۶۵-۷۰. ۱۸- Huang CF, Lin SS, Liao PH, Young SC, Yang CC. The immunopharmaceutical effects and mechanisms of herb medicine. Cell Mol Immuno. ۲۰۰۸ ۵(۱): ۲۳-۳۱. ۱۹- Tiron A, Vasilescu C. Role of the spleen in immunity. Immunologic consequences of splenectomy. Chirurgia. ۲۰۰۸ ۱۰۳(۳): ۲۵۵-۶۳. ۲۰- Bordbar N, Karimi MH, Amirghofran Z. The effect of glycyrrhizin on maturation and T cell stimulating activity of dendritic cells. Cell Immunol. ۲۰۱۲ ۲۸۰(۱): ۴۴-۹. ۲۱- Raphael TJ, Kuttan G. Effect of naturally occurring triterpenoidsglycyrrhizic acid, ursolic acid, oleanolic acid and nomilinon the immune system. Phytomedicine. ۲۰۰۳ ۱۰: ۴۸۳-۹. ۲۲- Soufy H, Yassein S, Ahmed AR, et al. Antiviral and immune stimulant activities of glycyrrhizin against duck hepatitis virus. Afr J Tradit Complement Altern Med. ۲۰۱۲ ۹(۳): ۳۸۹-۹۵. ۲۳- Kobayashi K, Kaneda K, Kasama T. Immunopathogenesis of delayed-type hypersensitivity. Microsc Res Tech. ۲۰۰۱ ۵۳(۴): ۲۴۱-۵. ۲۴- Zhang YH, Isobe K, Nagase F et al. Glycyrrhizin as apromoter of the late signal transduction of interleukin-۲production by splenic lymhocytes. Immunology. ۱۹۹۳ ۷۹: ۵۲۸-۵۳۴. ۲۵- Saxena S.Glycyrrhiza glabra:Medicine over the millennium. review article. J Nat Prod. ۲۰۰۵ ۴۵۴-۴۵۸. ۲۶- Teelucksingh S, Mackie AD, Burt D, McIntyre MA, Brett L,Edwards CR. Potentiation of hydrocortisone activityin skin by glycyrrhetinic acid. Immunol. ۱۹۹۰ ۳۳۵: ۱۰۶۰-۳. ۲۷- Schleimer RP. Potential regulation of inflammation in thelung by local metabolism of hydrocortisone. Am J Respir Cell Mol Biol. ۱۹۹۱ ۴: ۱۶۶-۷۳. ۲۸- Kroes BH, Beukelman CJ, Van Den Berg AJJ, Wolbink GJ, VanDijk H, Labadie RP. Inhibition of human complementby beta-glycyrrhetinic acid. Immunol. ۱۹۹۷ ۹۰: ۱۱۵-۲۰. ۲۹- Yang CL, Or TC, Ho MH, Lau AS. Scientific basis of botanical medicine as alternative remedies for rheumatoid arthritis. Clin Rev Allergy. ۲۰۱۳ ۴۴(۳): ۲۸۴-۳۰۰. ۳۰- Puchner A, Hayer S, Niederreiter B, et al. Effects of ۱۸β-glycyrrhetinic acid in hTNFtg mice – a model of rheumatoid arthritis. Wien Klin Wochenschr. ۲۰۱۲ ۱۲۴(۵-۶): ۱۷۰-۶. ۳۱- Song XH, Zan RZ, Yu CH, Wang F. Effects of modified HaizaoYuhu Decoction inexperimental autoimmune thyroiditis rats. J Ethnopharmacol. ۲۰۱۱ ۱۳۵(۲): ۳۲۱-۳۲۴. ۳۲- Zhou T, Deng X, Qiu J. Antimicrobial activity of licochalcone E against Staphylococcus aureus and its impact on the production of staphylococcal alpha-toxin. J Microbiol Biotechnol. ۲۰۱۲ ۲۲(۶): ۸۰۰-۵. ۳۳- Xiao Y, Xu J, Mao C, et al. ۱۸Beta-glycyrrhetinic acid ameliorates acute Propionibacterium acnes-induced liver injury through inhibition of macrophage inflammatory protein-۱alpha. J Biol Chem. ۲۰۱۰ ۲۸۵(۲): ۱۱۲۸-۳۷. ۳۴- Villinski JR, Bergeron C, Cannistra JC, et al. Pyrano-isoflavans from Glycyrrhiza uralensis with antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis. J Nat Prod. ۲۰۱۴ ۷۷(۳): ۵۲۱-۶. ۳۵- Snowden R, Harrington H, Morrill K, et al. A comparison of the anti-Staphylococcus aureus activity of extracts from commonly used medicinal plants. J Altern Complement Med. ۲۰۱۴ ۲۰(۵): ۳۷۵-۸۲. ۳۶- Long DR, Mead J, Hendricks JM, Hardy ME, Voyich JM. ۱۸beta-Glycyrrhetinic acid inhibits methicillin-resistant Staphylococcus aureus survival and attenuates virulence gene expression. Antimicrob Agents Chemother. ۲۰۱۳ ۵۷(۱): ۲۴۱-۷. ۳۷- Chen JC, Ho TY, Chang YS, Wu SL, Li CC, Hsiang CY. Identification of Escherichia coli enterotoxin inhibitors from traditional medicinal herbs by in silico, in vitro, and in vivo analyses. J Ethnopharmacol. ۲۰۰۹ ۱۲۱(۳): ۳۷۲-۸.

کلیدواژه ها:

Keywords: Glycyrhiza glabra L ، Humoral immunity ، Cellular immunity

نویسندگان

سید میثم ابطحی فروشانی

Dept. of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran

هادی اسمعیلی گورچین قلعه

Dept. of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran

رضا رضا پور

Dept. of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran

بهمن منصوری مطلق

Dept. of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran

علی روستایی

Dept. of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran