Effect of Berberine Hydrochloride and Vitamin E on Learning and Memory in Streptozotocin-Induced Diabetes in Rats

Background and Objective: Increasing evidence has shown that diabetes induces cognitive dysfunction and impairs learning and memory. Berberine is an isoquinoline alkaloid and vitamin E is a fat-soluble antioxidant with multiple pharmacological effects on diabetes. Thus, we investigated the effect of Berberine hydrochloride and vitamin E on diabetes-induced cognitive dysfunction in rats. Materials and Methods: ۴۸ male Wistar rats were randomly selected and allocated in ۶ control groups: control treated with vitamin E (۳۰mg/kg), diabetic and Berberine-treated diabetic group (۱۰۰mg/kg), vitamin E-treated diabetic group (۳۰mg/kg) and a diabetic group treated with both vitamin E and Berbrine. Diabetes was induced by STZ administration at dose of ۵۵mg/kg through Intraperitoneal injection route. Berberine hydrochloride and vitamin E were administered per os, respectively at doses of ۱۰۰ and ۳۰ mg/kg/day ۱ week after STZ injection for a period of ۶ weeks. Blood samples were taken from the tail vein ۱, ۳, ۵, ۷ weeks after STZ injection to measure blood glucose levels. Behavioral tests including spatial recognition and objective recognition were performed at the end of the study. Results: Diabetic group treated with both drugs demonstrated significant behavioral differences as compared to diabetic, vitamin E-treated diabetic (۳۰mg/kg), and Berberine -treated diabetic (۱۰۰mg/kg) groups. In the meantime, cognitive test value demonstrated an increase in this group. Conclusion: Berberine hydrochloride and vitamin E administration for ۶ weeks improve cognitive dysfunction in streptozotocin -induced diabetes in rats. References ۱- Pop-Busui۱ R, Sima A, Stevens M, Diabetic neuropathy and oxidative stress. Diabetes Metab Res Rev. ۲۰۰۶ ۲۲: ۲۵۷-۲۷۳. ۲- Galer BS, Gianas A, Jensen MP. Painful diabetic polyneuropathy: epidemiology, pain description, and quality of life. 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Keywords: Diabetes mellitus ، Berberine hydrochloride ، Vitamin E ، Spatial recognition ، Objective recognition