The Role of Heme Oxygenase -۱ System in Melanoma Tumor Growth of C۵۷Bl/۶ Mice

Background and Objective: Some evidence about the relationship between heme oxygenase and many cancers is available. Heme oxygenase has anti-apoptotic effects and contributes to tumor growth. The aim of this study was to evaluate the effect of heme oxygenase on melanoma tumor cells mitosis and tumor size in C۵۷BL/۶ mice. Materials and Methods: B۱۶F۱۰ melanoma cells were injected subcutaneously to ۱۸ C۵۷BL۶ mice with ۸ weeks of age. Mice were randomly divided into three groups: the first group received Zinc protoporphyrin (Znpp), heme oxygenase inhibitor (n = ۶), the second group, hemin (heme oxygenase activator) (n = ۶) and the third group((control group) (n = ۶)) received diluent injection. They received their injection every other day from the first day of study for ۱۶ days. Tumors were extracted in the ۱۶th day of study. Length and width of tumors were measured and mitotic cell activities were evaluated using immunohistochemistry staining and counting Ki-۶۷ posetive cells. Results: Our study results showed that size and weight of tumor and mitotic index in treated mice by Hemin were higher than two other groups. (P≤۰.۰۵). But mitotic index, tumor size and tumor weight in the recipient Znpp group and the control group were not statistically different. Conclusion: Stimulation of Hemeoxygenase-۱ pathway by hemin injection can increase melanoma tumor growth. However, inhibition of this pathway by Hemeoxygenase -۱ inhibitors may not be effective in reducing tumor growth and size. References ۱- Elwood JM, Jopson J. Melanoma and sun exposure: an overview of published studies. Int J Cancer. ۱۹۹۷ ۷۳: ۱۹۸-۲۰۳. ۲- Chang KW, Lee TC, Yeh WI, et al. Polymorphism in heme oxygenase-۱ (HO-۱) promoter is related to the risk of oral squamous cell carcinoma occurring on male areca chewers. Br J Cancer. ۲۰۰۴ ۹۱: ۱۵۵۱-۵. ۳- Halliwell B. Oxidative stress and cancer: have we moved forward? Biochem J. ۲۰۰۷ ۴۰۱: ۱-۱۱. ۴- Brigelius-Flohe R, Flohe L. 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Keywords: Melanoma ، Heme oxygenase ، Mitotic index